species accumulation curves and incidence-based species richness estimators to appraise the diversity of cultivable yeasts from beech forest soils物种积累曲线和incidence-based物种丰富度估计评价的多样性从山毛榉森林土壤可培养的酵母.pdfVIP

Species Accumulation Curves and Incidence-Based Species Richness Estimators to Appraise the Diversity of Cultivable Yeasts from Beech Forest Soils 1 1,2 1 Andrey M. Yurkov *, Martin Kemler , Dominik Begerow ¨ 1 Geobotany, Department of Evolution and Biodiversity of Plants, Faculty of Biology and Biotechnology, Ruhr-Universitat Bochum, Bochum, Germany, 2 Centre of Excellence in Tree Health Biotechnology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa Abstract Background: Yeast-like fungi inhabit soils throughout all climatic zones in a great abundance. While recent estimations predicted a plethora of prokaryotic taxa in one gram of soil, similar data are lacking for fungi, especially yeasts. Methodology/Principal Findings: We assessed the diversity of soil yeasts in different forests of central Germany using cultivation-based techniques with subsequent identification based on rDNA sequence data. Based on experiments using various pre-cultivation sample treatment and different cultivation media we obtained the highest number of yeasts by analysing mixed soil samples with a single nutrient-rich medium. Additionally, several species richness estimators were applied to incidence-based data of 165 samples. All of them predicted a similar range of yeast diversity, namely 14 to 16 species. Randomized species richness curves reached saturation in all applied estimators, thus indicating that the majority of species is detected after approximately 30 to 50 samples analysed. Conclusions/Significance: In this study we d