species boundaries and host range of tortoise mites (uropodoidea) phoretic on bark beetles (scolytinae), using morphometric and molecular markers物种界限和宿主范围的乌龟螨(uropodoidea)phoretic树皮甲虫(scolytinae),使用形态学和分子标记.pdfVIP

Species Boundaries and Host Range of Tortoise Mites (Uropodoidea) Phoretic on Bark Beetles (Scolytinae), Using Morphometric and Molecular Markers 1,2 ´ ´ 2 2 2 3 Wayne Knee *, Frederic Beaulieu , Jeffrey H. Skevington , Scott Kelso , Anthony I. Cognato , Mark R. Forbes1 1 Department of Biology, Carleton University, Ottawa, Ontario, Canada, 2 Canadian National Collection of Insects, Arachnids and Nematodes, Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada, 3 Department of Entomology, Michigan State University, East Lansing, Michigan, United States of America Abstract Understanding the ecology and evolutionary history of symbionts and their hosts requires accurate taxonomic knowledge, including clear species boundaries and phylogenies. Tortoise mites (Mesostigmata: Uropodoidea) are among the most diverse arthropod associates of bark beetles (Curculionidae: Scolytinae), but their taxonomy and host associations are largely unstudied. We tested the hypotheses that (1) morphologically defined species are supported by molecular data, and that (2) bark beetle uropodoids with a broad host range comprise cryptic species. To do so, we assessed the species boundaries of uropodoid mites collected from 51 host species, across 11 countries and 103 sites, using morphometric data as well as partial cytochrome oxidase I (COI) and nuclear large subunit ribosomal DNA (28S). Overall, morphologically defined species were confirmed by molecular datasets, with a few exceptions. Twenty-nine of the 36 uropodoid species (Trichouropoda, Nenteria and Uroobovella) collected in this study had narrow host ranges, while seven species had putative broad host ranges. In all but one species, U. orri, our data sup