黄体酮对大鼠试验性变应性脑脊髓炎治疗作用的研究-第三军医大学学报.docVIP

黄体酮对大鼠实验性变应性脑脊髓炎治疗作用的研究 余洪俊1,2,费军3,罗雪2，姚忠祥2（第三军医大学：1西南医院康复科, 2基础医学部组织学与胚胎学教研室，3大坪医院野战外科研究所创伤中心，重庆 400038） 摘要： 目的 研究黄体酮对实验性变应性脑脊髓炎（EAE）大鼠的治疗作用。方法 采用注射豚鼠脊髓匀浆的方法制作雄性Wistar大鼠EAE模型。当EAE大鼠出现神经症状后，开始在治疗组腹腔内注射溶于2.5ml芝麻油中的黄体酮（4mg/Kg/d,连续注射4d），模型组注射等量的芝麻油。分别对两组大鼠进行运动功能评分和脑组织的电镜检查、LFB染色、锇酸Marchi’s染色。染色结果采用IPP图像分析软件计算OD值。结果 治疗组和模型组神经功能评分无明显区别。但是在开始治疗后第6、10、25d，治疗组正常髓鞘的OD值明显高于模型组，而退变髓鞘的OD值明显低于模型组。结论 黄体酮有利于EAE大鼠中枢神经系统的髓鞘再生，促进髓鞘修复。 关键词：实验性变应性脑脊髓炎；黄体酮；髓鞘再生 中图法分类号: 文献标识码: The research of treating EAE rat with progesterone YU Hong-jun1，2,FEI Jun3,LUO Xue1,YAO Zong-xiang1(1Department of Histology and Embryology, 2Rehabilitation department of southwest hospital, 3Trauma center of daping hospital,Third Military Medical Uiniversity, Chongqing 400038, China) Abstract: Objective To study the treating effect of progesterone for experimental allergic encephalomyelitis（EAE）. Methods The male Wistar rats were immunized with guinea pig’s spinal cord.The progestrone was injected in Wistar rats’abdominal cavity. All the rats were evaluaion with the nerve function score. Their brains were examined by electron microscope and stained by LFB and Marchi method . The IPP system was used to calculate the OD value of normal and degenerative myelin. Results There was no obvious differention in the nerve function score between treating and exemplar group. But in 6th,10th,25th day after progesterone treating, the OD value of normal myelin of treating group were higher than that of exemplar. The OD value of degenerative myelin of treating group were lower than that of exemplar. Conclusion The progestrone is profit to remyelination of CNS. Key Word：experimental allergic encephalomyelitis；progesterone；remyelination 多发性硬化（multiple sclerosis ,MS））[1]，是造成MS患者的神经系统功能障碍逐渐加重的主要原因。因此，对MS的针对性治疗是促 进神经系统有效地进行髓鞘再生，修复髓鞘损伤[2]。 实验性变应性脑脊髓炎（experimental allergic encephalomyelitis,EAE）是目前公认的作为研究MS的动 物模型。本研究在EAE大鼠出现神经症状后开始黄体酮治 基金项目：国家自然科学基金面上项目，重庆市自然科学基金（2007BB5008）’s染色。 取材