互花米草入侵对树秋茄根际与根内细菌群落结构与多样性的.docx

互花米草入侵对红树秋茄根际与根内细菌群落结构与多样性的影响廖丹1黄华斌1庄峙厦1洪有为2**1厦门华厦学院检验科学与技术系厦门 3610242中国科学院城市环境研究所城市环境与健康重点实验室厦门 361021摘要根际/根内微生物在湿地生态系统中碳、氮、硫循环发挥着重要作用，也是外来植物入侵机制研究的重要组成部分。本研究选取福建九龙江口南岸互花米草纯林、秋茄纯林及互花米草和秋茄混交林等生境，采用Illumina高通量测序技术分析植物根内、根表及根际沉积物中细菌群落组成，结果发现：互花米草纯林根际细菌的多样性指数明显高于混交林和秋茄纯林的；基于UniFrac主成分分析(PCA)结果表明，不同生境各细菌群落组成存在显著性差异；两类盐生植物根表细菌以变形菌门(Proteobacteria)、拟杆菌门(Proteobacteria)、绿弯菌门(Chloroflexi)和厚壁菌门(Chloroflexi)为主，其中互花米草纯林中各类细菌占比分别为78.0%、5.6%、3.3%和1.6%；盐生植物根内以蓝细菌(Cyanobacteria)和变形菌门(Proteobacteria)等为主。线性判别分析(LDA)结果显示，盐生植物根内和根表存在大量与碳、氮、硫循环相关的功能微生物，如假交替单胞菌科(Pseudoalteromonadaceae)、弧菌科(Vibrionacea)和嗜甲基菌科(Methylophilaceae)和脱硫弧菌属(Desulfovibrio)等。总之，与根际微生物相比，根内细菌群落对植物入侵造成的环境变化更加敏感，将为深入研究互花米草入侵机制提供重要科学依据。(图5表2参36)关键词内生细菌；根际细菌；群落结构；互花米草；红树植物；Illumina测序CLC Q938.1收稿日期 Received: 接受日期 Accepted: 福建省中青年教师教育科研项目(JA15879)资助 Supported by the Young?Middle-aged Teacher?Education?Science Research?Foundation in Fujian Province, China (JA15879)**通讯作者 Corresponding author (E-mail: ywhong@iue.ac.cn).Diversity of endophytic and rhizoplane bacterial communities associated with exotic Spartinaalternifloraand native mangrove using Illuminaamplicon sequencingLIAO Dan1, HUANG Huabin1, ZHUANG Shixia1, HONG Youwei 2**1Department of Science and Technology for Inspection of Xiamen HuaxiaUniversity, Xiamen 3610242Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021AbstractObjectives:Root-associated microbial communities are very important for biogeochemical cycles of carbon, nitrogen and sulfur in wetland ecosystems and help to elaborate the mechanisms of plant invasions. In the estuary of Jiulong River (China), Spartinaalterniflorahas widely invaded Kandeliaobovata-dominated habitats, offering an opportunity to study the influence of root-associated bacteria. Methods: The community structures of endophytic and rhizosphere bacteria associated with selected plant species were investigated using the barcoded Illumina paired-end sequen