CLA对2型糖尿病大鼠肝脏线粒体呼吸链酶活性及氧化应激影响.docVIP

CLA对2型糖尿病大鼠肝脏线粒体呼吸链酶活性及氧化应激影响 　 作者：徐文，海春旭，杨晨，李嘉琳，王鹏 【关键词】 实验二型糖尿病 Effect of conjugated linoleic acids on hepatic oxidative stress and hepatic mitochondrial respiratory chain enzyme activities in type 2 diabetic rats 　　【Abstract】 AIM: To observe the effect of conjugated linoleic acids on hepatic oxidative stress and enzyme activities of hepatic mitochondrial respiratory chain in type 2 diabetic (DM2) rats. METHODS: Sixty rats were randomly divided into 3 groups: control group, diabetic (DM) group and conjugated linoleic acids (CLA) treatment group. DM2 rat model was induced by feeds of higher sugar, fat and cholesterol with the injection of streptozotocin. Blood glucose was determined by a blood glucose testing instrument and serum insulin by a radioimmunological method. The contents of malondialdehyde (MDA), reduced glutathione（GSH） and the activity of superoxide dismutase (SOD) and catalase (CAT) in hepatic tissues were tested by biochemical methods. The enzyme activities of hepatic mitochondrial respiratory chain were measured by Clark oxygen electrode. RESULTS: Compared with those in control group, the blood glucose, serum insulin and MDA in liver increased significantly (Plt;0.05) and GSH content, activities of SOD, and CAT as well as mitochondrial respiratory chain enzymes in the hepatic tissues decreased remarkably (Plt;0.05) in the DM group. In CLA treatment group, the indexes above ameliorated to different degree and these changes were in a dosedependent manner. CONCLUSION: CLA ameliorates the energy metabolism impairment of DM liver by suppressing the oxidative stress and improving the activity of enzymes in mitochondrial respiratory chain, and CLA has effective protection from the oxidative injury induced by DM. 　　【Keywords】 conjugated linoleic acids; type 2 diabetes; respiratory chain enzymes；oxidative stress 　　【摘要】 目的：观察共轭亚油酸(CLA)对2型糖尿病(DM2)大鼠肝脏线粒体呼吸链酶活性及肝内氧化应激（OS）的影响. 方法：将60只SD大鼠随机分为阴性对照组，DM组和CLA治疗组. 高糖、高脂及高胆固醇饲料诱导胰岛抵抗联合ip链脲佐菌素建立大鼠DM2模型. 血糖仪检测血糖浓度，放免