COL1A2基因多态性及儿童氟斑牙关系.docVIP

COL1A2基因多态性及儿童氟斑牙关系 　 作者：黄辉,贾新梅,李世宏,阎平平,程学敏,崔留欣,巴月 【关键词】 I型胶原；氟斑牙；基因多态性 　　摘要： 目的 调查I型胶原α2链（COL1A2）PvuⅡ基因型在饮水氟、碘浓度不同地区儿童中的分布，探讨COL1A2 PvuⅡ基因多态性与儿童氟斑牙的关系。方法 采用聚合酶链反应－限制性片段长度多态性（PCR-RFLP）方法检测氟碘正常区、单纯高氟区和高氟高碘区8～12岁儿童的COL1A2 PvuⅡ基因型。Dean氏法检查儿童氟斑牙患病情况。结果 3区儿童COL1A2 PvuⅡ基因型在氟碘正常地区分布频率分别为PP233%，Pp367%，pp400%；单纯高氟区分布频率分别为PP98%，Pp451%，pp451%，高氟高碘区分布频率分别为PP137%，Pp384%，pp479%，3区分布差异无统计学意义（χ2＝5191，Pgt;005）。儿童氟斑牙患病率分别为0，527%，507%，差异有统计学意义（χ2＝49081，Plt;005）；单纯高氟区和高氟高碘区氟斑牙患病率差异无统计学意义（χ2＝0060，Pgt;005），但高氟高碘区的氟斑牙指数低于单纯高氟区。结论 COL1A2 PvuⅡ基因多态性与所调查儿童氟斑牙无明显关系。 　　关键词： I型胶原；氟斑牙；基因多态性 　　Relationship of COL1A2 polymorphism to children’s dental fluorosis 　　Abstract： Objective To explore the distribution of collagen type 1 alpha2 (COL1A2)PvuⅡ genotype in children who lived in the high fluoride,high iodine areas,and evaluate the relationship of COL1A2 PvuⅡgene polymorphism to children’s dental fluorosis.Methods Polymerase chain reaction-restriction fragment length polymorphism technique（PCR-RFLP） was used to determine COL1A2 PvuⅡ genotype of the different children lived in the normal fluoride and iodine area ,the high fluoride area,the high fluoride and high iodine area.The incidence of dental fluorosis was examined by using Dean’s method.Results The distribution frequency of COL1A2 PvuⅡ genotype was PP23.3%，Pp36.7%，pp40.0% in the normal fluoride and iodine area,PP9.8%，Pp45.1%，pp45.1% in the high fluoride area ,and PP13.7%，Pp38.4%，pp47.9% in the high fluoride and high iodine area respectively.There were no significant differences in the three areas（χ2＝5.191，Pgt;0.05）.The prevalence of dental fluorosis in these three groups was 0，52.7%，50.7% respectively,and there were significant differences in the three areas（χ2＝49.081，Plt;0.05）.There was no significant difference of children dental fluorosis between the high fluoride area and the high fluoride and iodine area（χ2＝0.060，Pgt;0.05）,but the community fluorosis index(CFI) in the high fluoride and iodine area was lower than that in the high fluorid