Lewis全细胞肿瘤疫苗防治C57BL-6小鼠Lewis肺癌实验探究.docVIP

Lewis全细胞肿瘤疫苗防治C57BL/6小鼠Lewis肺癌实验探究 　【摘要】; 目的 探讨Lewis全细胞肿瘤疫苗对小鼠Lewis肺癌的防治作用。方法 用Lewis全肿瘤疫苗免疫C57小鼠4次,其中首次免疫用含弗氏佐剂油剂疫苗，第4次免疫的同时在小鼠右腋下接种Lewis肺癌细胞，定期观察小鼠成瘤情况及小鼠免疫状态变化。用流式细胞术检测疫苗组和对照组小鼠外周血CD4+T细胞，CD8+T细胞及CD19+B细胞百分比，以及CD4/CD8的变化。结果 疫苗组与对照组小鼠成瘤率、成瘤时间、肿瘤大小差异均无显著性；疫苗组与对照组相比，其外周血CD4+、CD8+T淋巴细胞亚群的百分比差异无显著性，CD4/CD8比值的差异也无显著性,疫苗组外周血CD19+B淋巴细胞百分比略高于对照组，差异有显著性（P=0.014）。结论 用Lewis肿瘤全细胞疫苗不能提高C57BL/6小鼠的T淋巴细胞免疫功能，可以提高其B淋巴细胞免疫，但不能使小鼠获得有效的免疫保护。 【关键词】; 肺癌；肿瘤细胞疫苗；流式细胞术；Lewis 肺腺癌细胞 　　Abstract:Objective To observe the tumor formation and the efficacy of immune response of C57BL/6 mice after the mice were inoculated an autologous tumorcell vaccine against Lewis lung carcinoma.Methods; Two groups of mice (vaccine group and control group) were inoculated, totally 4 times. When the third vaccine was inoculated, the mice were injected with Lewis lung tumor tissue suspension at right axillary subcutaneously region at the same time. Then tumor formation was observed. The subsets of the lymphocytes (CD4+T cell, CD8+T cell and CD19+B cell) in peripheral blood of the two groups of mice were also assayed with flow cytometry. Results Two groups of mice have the same rate of tumorformation (100%).And there is no significant difference in time of tumorformation and tumor volume between the two groups.Contrasted with the control group, the vaccine group shows no significant difference in the subsets of the lymphocytes in peripheral blood. Conclusion This study indicates that the Lewis autologous tumorcell vaccine can enhance Bcell but not Tcell immune responses and cannot protect mice from tumor challenge. 　　Key words:lung cancer; cancer vaccine; immunotherapy；mice 　　肺癌是严重危害人类健康的重大疾病，其发病率及病死率在国内外均呈逐年上升趋势。肺癌的治疗除手术、放疗和化疗外,近年的研究热点——肿瘤疫苗免疫治疗则被人们认为是治疗肺癌的第4种重要措施。肿瘤的主动特异性免疫治疗是免疫治疗中极具吸引力的治疗方法［1］。虽然国内外有关实验研究相继建立了一系列在动物模型中被证明有效的细胞瘤苗设计方案,但在临床上所取得的疗效有限［2,3］。因此肿瘤细胞疫苗是否能引起机体的特异性免疫反应，进而提高机体抗肿瘤效应，仍有待进一步的实验研究。本研究采用Lewis肺腺癌细胞株制成全细胞肿瘤疫苗，免疫C57小鼠后观察该肿瘤疫苗对C57小鼠的免疫防治效果。 　　1; 材料与方法 　　1.1; 材料; 　　1.1.1; 实动物及分组; 近交系C57BL/