Livin，Survivin及HPV16-18在宫颈癌及癌前病变中表达及相关性.docVIP

Livin，Survivin及HPV16/18在宫颈癌及癌前病变中表达及相关性 　 作者：施铮铮，朱华，郑飞云，胡燕，张虎祥，万丽 【摘要】 　　目的 ：研究宫颈上皮内瘤变（CIN）和宫颈癌中凋亡抑制因子Livin，Survivin的表达及HPV16/18感染情况，探讨它们在宫颈癌及癌前病变中的意义及相关性。方法:采用免疫组织化学SP法对81例宫颈癌、74例CIN、20例正常宫颈组织进行Livin的检测，应用原位杂交法进行Survivin及HPV16/18的检测。结果：Livin，Survivin在正常宫颈组织中几乎不表达，在CIN和宫颈癌中表达上调，并随CIN级别和肿瘤临床分期的升高、肿瘤分化程度的降低、伴有淋巴结转移而上升（P＜0.05）；而与宫颈癌的病理类型、肿块生长类型无相关性（P＞0.05）。HPV16/18在正常宫颈组织、CIN 和宫颈癌中感染率逐渐升高（P＜0.05），但与CIN级别、宫颈癌的临床分期、组织分化、病理类型、肿块类型、淋巴结转移无关（P＞0.05）。Survivin的表达与HPV16/18感染呈正相关；Livin的表达与Survivin呈正相关，但与HPV16/18感染不存在相关性。结论：Livin与Survivin的表达相似，在宫颈癌的发生、发展中可能有协同促进抗凋亡作用，并提示与肿瘤的恶性程度及不良预后有关；Survivin与HPV16/18对宫颈癌发生可能起协同作用。 【关键词】 凋亡抑制因子 Livin Survivin HPV16/18 CIN 宫颈癌 　　Abstract: Objective: To investigate the expression of Livin and Survivin which are apoptosis-profilin in CIN and cervical carcinoma， and detect the infection of HPV16/18， explore their clinical significance and correlatiuity in cervical carcinoma and its precancerous lesion. Methods: The expressions of Livin in 81 cases of cervical carcinoma， 74 cases of CIN and 20 cases of normal cervix were examined with SP immunohistochemistry. The expressions of Survivin mRNA and HPV16/18 DNA were examined with in situ hybridization. Results: Livin and Survivin were seldom expressed in normal cervical tissue，and were overexpressed significantly in CIN and cervical carcinoma tissue. The increased expression of Livin and and Survivin were related to the higher grades of CIN，and also related to the higher clinical stages， the lower histodifferentiation， and lymphnode metastasis in cervical carcinoma(P lt;0.05). They were not associated with pathological type and tumor growth type(P gt;0.05). The infection ratio of HPV16/18 was significantly higher in CIN and cervical carcinoma than that in normal cervical tissue， and there was a significant difference between the three (P lt;0.05). But it was not associated with the grade of CIN， clinical stage， histodifferentiation， pathological type， tumor growth type and lymphnode metastas