L┐NAME对新生鼠缺氧性脑损伤内源性NO及ET影响.docVIP

L┐NAME对新生鼠缺氧性脑损伤内源性NO及ET影响 　 作者：王玲，惠延平，侯英萍 ，鱼绥和 【关键词】 L-硝基-精氨酸甲酯 　　关键词: L-硝基-精氨酸甲酯;内皮素;一氧化氮;脑缺氧;新生鼠 　　摘 要:目的 用新生大鼠急性缺氧模型，探讨NO合成酶抑制剂L-硝基-精氨酸甲酯（L-NAME）在缺氧后脑损伤中的作用，进而探讨一氧化氮（NO）和内皮素（ET）在早期缺氧性脑损伤中的作用地位. 方法 检测正常对照组、缺氧组和缺氧前应用L-NAME预处理的新生大鼠血浆ET及脑组织匀浆NO含量及NOS的活性，并观察各组大鼠脑组织病理改变及毛细血管充盈不良程度. 结果 缺氧组血浆ET水平较正常对照组显著升高（Plt;0.01），而脑NO水平及NOS活性无显著变化（Pgt;0.05），脑毛细血管充盈不良程度与正常对照组相比明显加重（Plt;0.05）;L-NAME组血浆ET水平较缺氧组显著升高（Plt;0.05），脑NO含量及NOS活性较缺氧组显著下降（Plt;0.01），脑毛细血管充盈不良程度与缺氧组相比明显加重（Plt;0.05）. 结论 急性缺氧早期，ET异常增高是脑损伤的主要因素;此时抑制内源性NO的合成，可加重脑组织的微循环障碍而导致脑缺氧缺血性损害的进一步加重. Effect of L┐NAME on NO and 　　ET in newborn rats with hypoxic brain injuries 　　Abstract:AIM To explore the role of nitric oxide（NO）and endothelin（ET）in the early phase of hypoxic brain injuries by examing the effect of Nω -nitro-L-arginine methyl ester（L-NAME），an inhibitor of nitric oxide synthase（NOS），on brain NO production and serum ET level in newborn rats with hypoxic brain injuries.METHODS The rats were di-vided into normal control group，hypoxia group and L-NAME+hypoxia group.The ET level in serum，NO pro-duction，NOS activity，pathology and capillary perfusion in brain were investigated in3groups.RESULTS The serum ET level of hypoxia group was significantly higher than that of controls（Plt;0.01），but NO production and NOS activity were not significantly different between2groups（Pgt;0.05），and the brain capillary perfusion was more serious in hypoxia group that that in controls.Compared with the hypoxia group，the serum ET level of L-NAME+hypoxia group was increased significantly（Plt;0.05），NO production and NOS activity in brain was decreased significantly（Plt;0.01），and brain capillary perfusion was serious significantly（Plt;0.05）.CONCLUSION Abnormal increase of serum ET in early phase of acute hypoxia is the main factor of brain in-jury;NOS inhibitor L-NAME may make microcirculation dysfunction worse and lead to a further hypoxic-ischemic brain injury. 　　0 引言 　　一氧化氮（NO）和内皮素（ET）在围产期窒息时两者均升高，但它们在窒息后脑损伤中的作用及应用NO合成酶抑制剂的利弊尚存在争论.我们应用