VEGFR-3及VEGF-C在乳腺癌中表达及意义.docVIP

VEGFR-3及VEGF-C在乳腺癌中表达及意义 　【摘要】 目的 探讨VEGFR-3和VEGF-C在乳腺癌中的表达，分析表达结果与肿瘤临床分期及淋巴结转移的关系，为临床指导治疗及判断预后提供依据。方法 采用免疫组化SP法检测75例乳腺癌中的VEGFR-3和VEGF-C的表达。结果 75例乳腺癌组织中VEGFR-3 和VEGF-C阳性表达率分别为52.00％（39/75）、74.66％（56/75）；乳腺癌组织中VEGFR-3和VEGF-C阳性表达率在有、无淋巴结转移组之间的差异均有显著性（Plt;0.05）；VEGFR-3和VEGF-C在乳腺癌中的阳性表达率与临床分期呈正相关；微淋巴管计数在VEGFR-3表达阳性组和阴性组之间的差异有显著性（Plt;0.01）。结论 VEGFR-3和VEGF-C在乳腺癌中的表达与肿瘤临床分期及淋巴结转移的关系密切。 【关键词】 乳腺癌；血管内皮生长因子受体-3；血管内皮生长因子-C；淋巴结转移 【Abstract】 Objective To investigate the expression of VEGFR-3 and VEGF-C in breast cancer ,and explorer their correlation to different clinical TNM stages and lymphatic metastasis,for providing evidence for clinical treatment and prognosis.Methods The expression of VEGFR-3 and VEGF-C in 72 cases of breast cancer were respectively detected by SP immunohistochemistry.Results The positive rates of VEGFR-3 and VEGF-C in 75 samples of breast cancer were 52.00％and 74.66% respectively ;The positive expression rates of VEGFR-3 and VEGF-C in breast cancer with lymph node metastasis were significantly higher than that without lymph node metastasis（Plt;0.05）;The expression of VEGFR-3 and VEGF-C in breast cancer were positively correlated with clinical TNM stages;There is significantly difference between the VEGFR-3-positive group and negative group in the counts of lymph vessels（Plt;0.01）.Conclusion The expressions of VEGFR-3 and VEGF-C in breast cancer have close relations with lymph node metastasis and clinical TNM stages. 【Key words】 breast cancer; VEGFR-3; VEGF-C; lymph node metastasis 乳腺癌是我国女性最常见的恶性肿瘤之一，也是严重威胁女性健康的重要疾病，而淋巴结转移是影响乳腺癌患者预后的重要因素,故乳腺癌的淋巴管的生成和淋巴结的转移是近年来研究的热点之一。已有的研究表明，血管内皮生长因子-C（ vascular endothelial growth factor C, VEGF-C）是淋巴管内皮细胞增殖和趋化的特异诱导因子，血管内皮生长因子受体3（ vascular endothelial growth factor receptor 3, VEGFR-3） 是VEGF-C受体，VEGF-C和VEGFR-3可以诱导肿瘤内部新生淋巴管形成和肿瘤组织周围淋巴管扩张，其表达强度与淋巴结转移以及预后有密切关系［1］。本文通过检测75例乳腺癌组织中VEGFR-3、VEGF-C两种蛋白表达和微淋巴管计数，探讨它们与乳腺癌的临床分期及淋巴结转移的关系，为临床指导治疗及判断预后提供依据。 　　1 资料与方法 1．1 临床病理资料 所有材料取自2000年1月～2004年8月在我院行根治术的乳腺癌标本，全部标本均经10％甲醛固定，常规取材后经