VEGF及E-cadherin在胃癌中表达及意义.docVIP

VEGF及E-cadherin在胃癌中表达及意义 　 作者：罗秉庆，郑绍光，唐青梅，龚广 【摘要】 目的 探讨血管内皮生长因子（VEGF）和钙粘附分子（E-cadherin，E-cad）在胃癌中的表达及意义。方法 应用核酸原位杂交方法检测62例胃癌组织中VEGF和E-cad mRNA表达情况。结果 62例胃癌中VEGF和E-cad mRNA阳性表达率分别为90.32％（56/62 ）和32.26％（20/62 ），VEGF mRNA在胃癌中的阳性表达率显著高于正常胃粘膜上皮组织；E-cad mRNA阳性表达率显著低于正常胃粘膜上皮组织(Plt;0.01)。VEGF mRNA高表达和E-cad mRNA低表达与胃癌组织分化、TNM分期、淋巴结转移有关(P＜0.01)。胃癌中VEGF和E-cad mRNA表达呈负相关(P＜0.01)。结论 VEGF mRNA过表达与 E-cad mRNA失活可加速胃癌的浸润转移。VEGF和E-cad mRNA检测可作为评价胃癌的恶性程度和判断预后的重要指标。 【关键词】 胃肿瘤；肿瘤转移；血管内皮生长因子；钙粘附分子；原位杂交 　　Abstract： Objective To assay vascular endothelial growth factor (VEGF) and E-cadherin (E-cad) expression and outline their clinical significances. Methods A situ hybridization was used to detect the expression of VEGF and E-cad mRNA in 62 patients with stomach cancer. Results The positive rates of VEGF and E-cad mRNA in 62 sample tissues of stomach cancer were 90.32% (56/62) and 32.26% (20/62).The VEGF mRNA staining was more strong in stomach cancer cells than normal gastric mucosa，and the E-cad mRNA staining was weaker (Plt;0.01).The high expression of VEGF mRNA and the low expression of E-cad mRNA are positively correlated with the differentiation of cancer tissues，the TNM stage and the metastasis of lymph node (Plt;0.01).In stomach cancer，the expression of VEGF mRNA is adversely associated with E-cad mRNA expression (Plt;0.01). Conclusion Over expression of VEGF mRNA and inactivation of E-cad mRNA enhance the infiltration and metastasis of stomach cancer.Detecting of VEGF and E-cad mRNA can be served as significant markers for evaluating the malignance and prognosis of stomach cancer. 　　Key words： stomach neoplasms; neoplasm metastasis; vascular endothelial growth factors; E-cadherin; in situ hybridization 　　癌的侵袭转移是多阶段的复杂过程，肿瘤新生血管的形成和细胞粘附系统的崩溃导致癌细胞脱离母癌是始动侵袭转移的关键［1，2］。血管内皮生长因子(VEGF)能促进新生血管的形成，肿瘤新生血管的形成在为肿瘤细胞生长提供养分时，也为肿瘤侵袭转移创造了条件，而肿瘤新生血管一旦被启动，便会无限制地增生且不受机体制约［3］。钙粘附分子(E-cadherin)代表一组参与细胞间或细胞与基质间作用的细胞表面分子，其表达丧失会导致肿瘤细胞脱离癌灶，启动转移和浸润［4］。我们应用核酸原位杂交技术，检测胃癌中VEGF和 E-cad mRNA的表达水平，探讨它们对胃癌生物学行为的