中药醇沉工艺颗粒沉降过程初步探究.docVIP

中药醇沉工艺颗粒沉降过程初步探究 　 作者：李页瑞，刘雪松，王龙虎，金胤池，陈勇 【摘要】 目的对中药醇沉工艺中颗粒沉降过程及其特点进行研究，初步了解不同种类中药醇沉过程中沉降颗粒的基本特点及其关键工艺参数，掌握沉降过程的规律，为醇沉工艺设计及工业化应用提供指导。方法以丹参、苦参和枳壳3种药材为研究对象，采用紫外分光光度法分别测定3种药材提取液醇沉过程中有效成分的保留量，同时测定了沉降颗粒含量、颗粒粒度和沉降速度等参数。结果不同药材有效成分保留量在醇沉过程中都有不同程度的下降;沉降颗粒含量随着静置时间的延长而增加;丹参、苦参和枳壳沉降颗粒的平均粒径分别为36.272，131.820 和0.684 μm，最小粒径分别为2.000，6.325和0.142 μm，平均沉降速率分别为5.04×10-4，1.95×10-4和1.63×10-7 m·s-1。结论中药醇沉时，形成的杂质颗粒有两种沉降方式：一种是自由沉降，另一种是絮凝沉降。应根据不同种类中药醇沉颗粒的沉降过程及其特点进行工艺设计。 【关键词】 中药; 醇沉工艺; 颗粒沉降过程; 沉降方式; 紫外分光光度法 　　Abstract：ObjectiveTo study the process of particle sedimentation of alcohol precipitation process of traditional Chinese medicine and its characteristics, and to preliminarily understand the basic characteristics and key technological parameters of the settling particle in the alcohol precipitation process of different kinds of traditional Chinese medicines, and hold the regulation of sedimentation and providing guidance for technology design and industrial application.MethodsSalvia Miltiorrhiza, Radix Sophorae Flavescentis and Fructus Aurantii were used as the experimental materials and UV spectrophotometry was used to determine the effective components of the solution in the process of alcohol precipitation, parameters like the content of settling particulate, the particle size and the sedimentation velocity were also determined. Results The content of the effective components was lower than the original in different levels during the precipitation process. The content of the sedimentation particle increased with the extension of the settling time. The average diameters of particles in each material solution were 36.272 μm, 131.820 μm and 0.684 μm and the minimum diameters were 2.000 μm, 6.325 μm and 0.142 μm respectively, the average sedimentation velocity were 5.04×10-4m·s-1, 1.95×10-4m·s-1 and 1.63×10-7m·s-1. ConclusionThere are two ways for the particulate to sediment in the alcohol precipitation process in traditional Chinese medicine, one is free sedimentation, the other is floc