丹参在大鼠恶性梗阻性黄疸时对肿瘤细胞凋亡作用.docVIP

丹参在大鼠恶性梗阻性黄疸时对肿瘤细胞凋亡作用 　【关键词】 胆汁淤积；浸润和转移；增殖细胞核抗原；丹参；凋亡指数 【Abstract】 AIM： To establish malignant biliary obstruction models in SD rats, and to study the effects of Danshen injection on the growth of hepatocellular carcinoma, morphological change and PCNA expression and apoptosis index in carcinoma and pericarcinoma tissues. METHODS： ① Malignant biliary obstruction model in SD rats was made by vaccination of transplanted tumor in liver nearby the porta hepatis with Walker256 hepatocarcinoma cell line, which infiltrated proximal common bile duct to cause its narrowness and obstruction. ② The SD rats were divided into 4 groups that were treated ip by 0.9% normal saline (NS) (n=16), by inosine+vitamin C (n=26), by Danshen (n=34) and by 5FU (n=30) respectively. ③ The diameter of transplanted tumor, morphological change and the expression of PCNA and apoptosis index in carcinoma and pericarcinoma tissues were observed. ④ Statistical analysis was made in the experimental data. RESULTS: In Danshen group as compared with NS group and inosine+vitamin C group, the average size of hepatocarcinoma was reduced significantly, the tumor inhibitory rate and the metastasisinhibitory rate were higher obviously (Plt;0.01), PCNA expression was lower (Plt;0.01), apoptosis index was higher (Plt;0.01); while compared with 5FU group, the average size was significantly larger (Plt;0.05) but the metastasisinhibitory rate was lower evidently (Plt;0.05), apoptosis index was higher in carcinoma tissues (Plt;0.05). CONCLUSION： Danshen shows a protective effect against morphological change in SD rat models of malignant biliary obstruction, and exerts an inhibitory effect on the growth of hepatocarcinoma through suppressing the proliferation, increasing the apoptosis of the hepatocarcinoma cells. 【Keywords】 cholestasis; infiltration and metastasis; proliferating cell nuclear antigen; Danshen; apoptosis index 【摘要】 目的： 建立恶性梗阻性黄疸模型，通过对模型鼠腹腔内注射丹参注射液，观察其对肝癌瘤体大小、形态学改变和肝癌、癌周组织中增殖细胞核抗原（PCNA）的表达和肿瘤细胞凋亡表现，