丹参对肝癌致鼠梗阻性黄疸时VEGF表达影响.docVIP

丹参对肝癌致鼠梗阻性黄疸时VEGF表达影响 　【关键词】 肝肿瘤 Effect of Danshen injection on VEGF expression in SD rats with malignant obstructive jaundice caused by hepatocellular carcinoma 　　【Abstract】 AIM: To establish malignant biliary obstruction model of SD rats and to study the effects of Danshen injection on the tumor size, inhibition rates and metastasis rates of hepatocellular carcinoma and the expression of vascular endothelia cell growth factor (VEGF) in carcinoma and pericarcinoma tissues, adjacent lobe (leftinternal lobe) as well as lung tissues. METHODS: Malignant biliary obstruction models of SD rats were made by vaccination of transplanted tumor in liver near the porta hepatis with walker256 hepatocarcinoma cell line, which infiltrated and metastasized to proximal common bile duct and caused its narrowness and obstruction. The SD rats were divided into 4 groups: 0.9% NS (n=24), Inosine+VC (n=40), Danshen (n=40) and 5FU (n=40) respectively. The diameter of transplanted tumor, inhibition rates and metastasis rates of hepatocellular carcinoma and the expression of VEGF in carcinoma and pericarcinoma tissues, adjacent lobe (leftinternal lobe) as well as lung tissues were observed. Statistical analysis was made. RESULTS: The diameter of the tumor was reduced and the inhibition rates and metastasis rates of hepatocellular carcinoma were upgraded after treatment of Danshen injection compared with those in the rats treated by 0.9% NS. and Inosine combined with VC (Plt;0.01), but the diameter of tumor was slightly downgraded after treatment of Danshen injection compared with that in the rats treated by 5FU (Plt;0.01). The inhibition rates（41.7%） and the liver metastasis rate (59.7% and 81.1%) of hepatocellular carcinoma in rats treated by Danshen injection were similar with those in the rats treated by 5FU (Plt;0.01). The expressions of VEGF in carcinoma and pericarcinoma tissues were significantly downgraded after treatment of Danshen injection compared with those in the rats treated