儿茶素对5-6肾切除大鼠肾脏微血管保护作用及其机制.docVIP

儿茶素对5/6肾切除大鼠肾脏微血管保护作用及其机制 　 作者：曹艳, 何小解, 向伟, 易著文 【摘要】 目的：探讨儿茶素对5/6肾切除大鼠血管紧张素转化酶（angiotensinconverting enzyme, ACE）、血管紧张素Ⅱ（angiotensin Ⅱ, Ang Ⅱ）及肾脏组织微血管密度（microvessel density, MVD）的影响。方法：60只SD大鼠被分为假手术组、模型组和儿茶素组。模型组和儿茶素组大鼠切除5/6肾脏制作模型，假手术组不做肾切除。儿茶素治疗8周与12周后，应用免疫组织化学法检测CD34标记的肾局部微血管数并计算密度；紫外分光光度法检测血浆与肾皮质中ACE活性；放射免疫法测定血浆与肾皮质中Ang Ⅱ浓度；苏木精和伊红（hematoxylin and eosin，HE）染色及过碘酸希夫（periodic acidSchiff, PAS）染色后，半定量积分法评价肾小球硬化指数(glomerular sclerosis index, GSI)与肾小管间质损伤指数(tubule interstitial score, TIS)。结果：儿茶素组GSI与TIS在第8、12周末均显著低于模型组（Plt;0.05，Plt;0.01）；模型组和儿茶素组肾小球及小管周围MVD在第8周与第12周末较假手术组均明显降低（Plt;0.01）；儿茶素组血浆、肾皮质中ACE活性与Ang Ⅱ浓度在实验第8与第12周末均显著低于模型组（Plt;0.01）；Pearson相关分析发现，MVD与GSI、TIS、ACE、Ang Ⅱ呈负相关（Plt;0.01），ACE、Ang Ⅱ与GSI、TIS呈正相关（Plt;0.01）。 结论：儿茶素可有效阻止5/6肾切除大鼠肾脏微血管密度的减少，抑制肾小球硬化与肾间质纤维化的进展，这可能与儿茶素抑制ACE的活性，减少Ang Ⅱ的生成有关。 【关键词】 儿茶素; 微血管密度; 血管紧张素Ⅱ; 血管紧张素转化酶; 大鼠 Objective: To investigate the effects of catechin on angiotensinconverting enzyme (ACE) activity, angiotensin Ⅱ(Ang Ⅱ) content and microvessel density (MVD) in renal tissues of 5/6 nephrectomized rats. Methods: Sixty SD rats were divided into 3 groups: shamoperated group, untreated group and catechin group. Animal model was reproduced by 5/6 nephrectomy. After 8 and 12week administration, rats were sacrificed. Renal MVD was measured by immunohistochemical method with CD34 marking. Activities of ACE in plasma and renal cortices were measured by ultraviolet spectrophotometer, and Ang Ⅱ contents in plasma and renal cortices were measured by radioimmunoassay. Glomerular sclerosis index (GSI) and tubule interstitial score (TIS) were calculated by semiquantitative integration with hematoxylin and eosin staining and periodic acidSchiff staining. Results: After 8 and 12week administration, the GSI and TIS in the catechin group were much less than those in the untreated group (Plt;0.05, Plt;0.01). MVDs around glomerulus and tubule at the end of the 8th and 12th week in the untreated group and the catechin group were much less than th