a bayesian method for calculating real-time quantitative pcr calibration curves using absolute plasmid dna standards贝叶斯方法计算实时定量pcr校正曲线使用绝对的质粒dna的标准.pdfVIP
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a bayesian method for calculating real-time quantitative pcr calibration curves using absolute plasmid dna standards贝叶斯方法计算实时定量pcr校正曲线使用绝对的质粒dna的标准
BMC Bioinformatics BioMed Central
Methodology article Open Access
A Bayesian method for calculating real-time quantitative PCR
calibration curves using absolute plasmid DNA standards
1 2 2 2
Mano Sivaganesan* , Shawn Seifring , Manju Varma , Richard A Haugland
and Orin C Shanks1
Address: 1U.S. Environmental Protection Agency, Office of Research and Development, National Risk Management Research Laboratory, 26 West
Martin Luther King Drive, Cincinnati, OH 45268, USA and 2U.S. Environmental Protection Agency, Office of Research and Development, National
Exposure Research Laboratory, 26 West Martin Luther King Drive, Cincinnati, OH 45268, USA
Email: Mano Sivaganesan* - sivaganesan.mano@; Shawn Seifring - siefring.shawn@; Manju Varma - varma.manju@;
Richard A Haugland - haugland.rich@; Orin C Shanks - shanks.orin@
* Corresponding author
Published: 25 February 2008 Received: 15 August 2007
Accepted: 25 February 2008
BMC Bioinformatics 2008, 9:120 doi:10.1186/1471-2105-9-120
This article is available from: /1471-2105/9/120
© 2008 Sivaganesan et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: In real-time quantitative PCR studies using absolute plasmid DNA standards, a
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