地鳖纤溶蛋白对荷S180及H22小鼠抑瘤作用探究.docVIP

地鳖纤溶蛋白对荷S180及H22小鼠抑瘤作用探究 　 作者：刘浩, 韩雅莉, 丁鸿, 黎子蔚 【摘要】 　　目的研究地鳖虫纤溶活性先导蛋白（EFP）对荷S180和H22小鼠肿瘤的抑制作用。方法通过体外实验，构建荷瘤小鼠模型，药物实验分为阳性对照组（环磷酰胺），阴性对照组（生理盐水），药物高浓度组、中浓度组和低浓度组。实体瘤通过瘤重计算抑瘤率，腹水瘤通过细胞浓度计算抑瘤率。结果EFP对S180腹水瘤有较好的抑制率， 药剂量为2.90mg/kg的抑制率为69.56%，明显高于环磷酰胺(20mg/kg)的51.75%的抑制率；EFP对S180实体瘤有较好抑制作用，药剂浓度为1.45 g/kg时，抑制率为37.48%，高于环磷酰胺(20 mg/kg)的35.59%的抑制率，并呈现一定的药物剂量依赖性；药剂浓度为0.73 mg/kg时，对H22实体瘤抑瘤率为41%，高于环磷酰胺(20 mg/kg)的37%的抑制率。结论EFP对荷S180和H22小鼠肿瘤有较好的抑制作用。 【关键词】 地鳖虫纤溶活性先导蛋白； S180细胞； H22细胞； 抑瘤作用 　　The Antitumour Effect of Eupolyphaga Fibrinolyric Protein on S180 and H22 in vivo 　　LIU Hao, HAN Yali, DING Hong, LI Ziwei 　　(Faculty of Light and Chemical Industry,Guangdong University of Technology,Guangzhou 510006, China; Department of Biology,Jieyang College,Jieyang 522000, China) 　　Abstract：ObjectiveTo investigate the antitumor effect of Eupolyphaga fibrinolyric protein on S180 and H22 in vivo. MethodsTumor-bearing mouse model was built in vivo, and the experiments were divided into positive control group (cyclophosphamide), negative control group (saline), the high concentration drug group, the middle concentration drug of group, the low concentration of drug group .The inhibition rate against ascites tumor was calculated by the tumer weights and the rate anainst solid tumor was calculated by the concentration of cells．ResultsEFP had significant inhibition against S180 ascites tumor, when the dose of drug was 2.90 mg/kg, the inhibition rate was 69.56%, higher than that of cyclophosphamide (20 mg/kg) (51.75%). EFP had preferably inhibition against S180 solid tumors, when the dose of drug was 1.45 mg/kg, the inhibition rate was 37.48%, higher than that of cyclophosphamide (20 mg/kg) (35.59%), and a certain dose-dependent .When the drug concentration was 0.73 mg/kg, the H22 solid tumor inhibition rate was 41%, higher than that of cyclophosphamide (20 mg/kg) (37%).ConclusionEFP has obvious antitumor effect on S180 and H22 in vivo. 　　Key words：Eupolyphaga fibrinolyric protei