增强红花黄色素A抗血栓作用生物转化机理探究.docVIP

增强红花黄色素A抗血栓作用生物转化机理探究 　作者：徐春，高陪，沈竞，苏建，杨琼木，孙启玲 【摘要】 　　目的对地衣芽孢杆菌（C213）发酵红花黄色素A（SYA）增强抗血栓作用的转化机理进行研究。方法高效液相色谱（HPLC）对SYA发酵前后的物质变化进行检测，用聚酰胺和制备型高效液相色谱对新物质进行分离并初步鉴定其结构。抗血栓作用是通过大鼠尾部血栓实验、凝血酶原时间（PT）、部分凝血酶活时间(APPT)等药效实验测定。结果发酵后生成的新物质（命名为SYAX）的抗血栓作用较SYA显著增强；其紫外吸收峰为353.6 nm，红外图谱中没有苯环峰出现，质谱显示其分子量为543。结论SYA通过C213的发酵，其抗血栓作用得到明显提高；该过程中，SYA被转化为一种新的高效抗栓成分SYAX。 【关键词】 生物转化 红花黄色素A 地衣芽孢杆菌 抗血栓 　　Abstract:ObjectiveTo study the substance foundation of fermentation of Flos Carthami with Bacillus licheniformis to improve its anti-thrombosis effect. MethodsBacillus licheniformis C2-13 was isolated from the aqueous compartments of slaughterhouse and used for the fermentation of Flos Carthami. The study of tail thrombosis, prothrombin time (PT) and activated partial thrombplastin time (APTT) were performed to measure the anti-thrombosis effect. High performance liquid chromatography (HPLC) was used to detect the product of fermentation and polyamide resin and preparative HPLC were used to isolate the new substance - SYA-X. Then Ultraviolet (UV), Infrared (IR) and Mass Spectrometry (MS) were used to analyze SYA-X. ResultsThe study of anti-thrombosis indicated that the anti-thrombosis effect of Flos Carthami was significantly improved after fermentation. The HPLC analysis indicated the effective compound of Flos Carthami-safflor yellow A (SYA, tR = 32.64 min) disappeared and a new substance SYA-X （tR＝23.82 min） formed during the fermentation. SYA-X was isolated from the fermentation broth. The peak value of SYA-X of UV went to 353.6 nm, IR did not have phenyl (1604,1505 cm-1) or phenyl syntonic trans -Alkene (1624,927 cm-1） and molecular ion peak was 543. These indicated the phenolic hydroxyl group of SYA disappeared and an acetyl formed in SYA-X. ConclusionFlos Carthami was fermented by C2-13 and its anti-thrombosis effect was improved. During the fermentation, the effective compound of Flos Carthami-safflor yellow A (SYA) was transformed into a new effective substance for thrombosis. 　　Key w