复合预处理对大鼠肠缺血再灌注保护作用.docVIP

复合预处理对大鼠肠缺血再灌注保护作用 　【关键词】 小肠 　　Protective effect of compound preconditioning on small intestine from ischemiareperfusion injury in rats 　　【Abstract】 AIM: To investigate the protective effect of compound precondition(ischemicpreconditioning under the mild hypothermia MHIP) on small intestine from ischemiareperfusion(I/R) injury in rats and its mechanism. METHODS: Thirtytwo rats were randomized into 4 groups (8 in each group): Sham operated group (sham), ischemiareperfusion (I/R) group, ischemicpreconditioning (IP) group, mild hypothermia ischemicpreconditioning (MHIP) group.The wet/dry ratio, Ca2+Mg2+ATPase in intestine tissue, the LDH, malondialdehyde (MDA), and activities of superoxide dismutase (SOD) and total antioxidase (TAX) in blood were compared. ultrastructure, Bcl2 and Bax expression in intestinal mucosa tissue were observed. RESULTS: After I/R, the tissue wet/dry ratio, LDH and MDA were significantly elevated（Plt;0.01）. Ca2+Mg2+ATPase, SOD, TAX declined obviously; the optic density of Bcl2 and Bax proteins increased significantly（Plt;0.01）. The tissue wet/dry ratio, LDH, MDA and the optic density of Bax protein were significantly lower in IP group than in I/R group, and in MHIP group than in IP group. The content of Ca2+Mg2+ATPase. SOD, TAX and the optic density of Bcl2 protein were significantly higher in IP group than in I/R group (Plt;0.01), and in MHIP group than in IP group （Plt;0.05）.CONCLUSION: MHIP can protect intestine against I/R injury in rats, which may be related to enhancing oxidationresistance of intestine, inhibiting lipid peroxidation, upregulating the protein expression of bcl2 genes and downregulating the protein expression of bax genes and reducing myocyte apoptosis. 　　【Keywords】 intestine, small; reperfusion; mild hypothermia; ischemicpreconditioning; gene expression 　　【摘要】 目的： 探讨复合预处理（亚低温下缺血预处理）对大鼠小肠缺血再灌注损伤的保护作用及机制. 方法： 将32只大鼠随机分为4组（每组8只）：假手术对照组（Sham）、缺血再灌注组（I/R）、缺血预处理组（IP）、亚低温预处理组（MHIP）. 比较各组小肠组织湿干质量比、Ca2+Mg2+ATP酶含量及血清乳酸脱氢酶（LDH）、超氧化物