太子参挥发油化学成分探究.docVIP

太子参挥发油化学成分探究 　【摘要】 　　目的比较不同挥发油提取方法对太子参挥发油化学成分的影响。方法提取挥发油采用方法I是乙醚提取，提取物经水蒸气蒸馏提取法；方法II是直接采用水蒸气蒸馏提取法。运用GC-MS-DS联用分析技术和气相色谱保留指数法，比较分析方法I和方法II太子参挥发油化学成分，用GC面积归一法分别计算了各成分的相对含量。结果方法I和方法II太子参的挥发油得率分别为0.28%和0.13%。从方法I提取的挥发油中鉴定出9种化合物，有7种化合物出峰的保留时间在30 min后，其中以邻苯二甲酸二丁酯相对含量最高（87.19%）；方法II鉴定8种化合物，均在30 min前出峰，其中以2-糠醇相对含量最高（79.76%）。显然，方法I和方法II提取的挥发油化学成分含量和组成有较大的差别；方法I提取的挥发油中未能检出2-糠醇，2-糠醇是太子参在水蒸气蒸馏过程中生成的，重复实验进一步验证该结果。结论不同提取方法对太子参挥发油化学成分含量和组成有明显的影响；2-糠醇不是太子参的天然成分。 【关键词】 太子参； 挥发油； 化学成分； 2-糠醇； 不同提取方法 　　Abstract：ObjectiveTo compare the effects of two different extraction methods on the chemical contents and components of the essential oils from Radix Pseudostellariae (RP)．MethodsMethod I :the essential oils were extracted from RP by diethyl ether, and then by stream distillation. Method II :the essential oils were immediately extracted from RP by stream distillation. The chemical components were analyzed by means of GC-MS-DS．The relative content of each component was calculated by area normalization．ResultsThe yields of essential oils extracted from RP by method I and method II were 0.28% and 0.13%, respectively. Nine chemical structures were identified in the essential oils from RP by method I. The relative time of 7chemical compounds were after 30min. Phthalic acid dinbutyl ester was the highest relative content (87.19%) among these chemicals. Eight chemical structures in the essential oils from RP by method II, and all relative time of them were before 30min. Among these compounds, 2-furanmethanol content was the highest relative content (79.76%). Obviously, the chemical contents and components in the essential oils from RP by the method I and the method II were significantly different. There was no 2-furanmethanol in the essential oils extracted from RP by method I. 2-furanmethanol was produced during stream distillation. This result was further proved with repeated test. ConclusionThe two different extract methods have significant effect on the chemical contents and components of