大黄灵仙胶囊对慢性肝损伤后胆结石形成干预作用探究.docVIP

大黄灵仙胶囊对慢性肝损伤后胆结石形成干预作用探究 　 作者：唐乾利，俞渊，黄名威，关中正，高善辉 【摘要】 目的探讨大黄灵仙胶囊对慢性肝损伤家兔胆结石形成的干预作用及其作用机制。方法将46只家兔随机分为正常组、慢性肝损伤组、肝硬化组、大黄灵仙胶囊大剂量组、大黄灵仙胶囊小剂量组。采用皮下注射CCl4方法进行慢性肝损伤造模,各组同时进行不同处理。最后观察各组胆囊成石率，肝脏功能生化指标，肝组织病理改变、超微结构改变，胆汁成分改变。结果①慢性肝损伤组、肝硬化组胆石形成阳性检出率较正常对照组显著增加（P＜0.05或P＜0.01）。大黄灵仙胶囊大剂量组、大黄灵仙胶囊小剂量组胆石阳性检出率较肝硬化组有显著降低(P＜0.01或P＜0.05)。②各模型组中肝脏超微结构均发生不同程度异常改变，大黄灵仙胶囊大、小剂量治疗组肝脏病理改变、肝细胞超微结构改变较模型组比较均有改善，大剂量组尤为明显。③大黄灵仙胶囊大、小剂量组血清AST，ALT，γ-GT，非结合胆红素均明显低于肝硬化组（P＜0.05或Plt;0.01）；总胆红素、间接胆红素、钙离子大黄灵仙胶囊大、小剂量组较肝硬化组明显降低（Plt;0.01），大剂量组较小剂量组明显降低（Plt;0.01）。结论大黄灵仙胶囊对慢性肝损伤后胆结石形成具有明显的预防效果，其作用机制主要与抑制CCl4所致肝脏损伤的作用有关，同时与大黄灵仙胶囊在多层次、多途径调节参与结石形成各因素关系密切。 【关键词】 慢性肝损伤； 肝硬化； 大黄灵仙胶囊； 胆结石； 肝脏超微结构 　　Abstract：ObjectiveTo study the interference effect and its mechanism of Dahuanglinxian Capsules on gallstone formation after chronic liver injury of rabbits. Methods46 rabbits were randomly divided into 5 groups, respectively named by group A (normal control group), group B (chronic liver injury group), group C (liver cirrhosis group), group D (group fed with large dose Dahuanglinxian capsule) and group E (group fed with large dose Dahuanglinxian capsule). The chronic liver injured rabbits were induced by subcutaneous injection with CCl4 and each group was treated by different factors at the same time. The gallstone formation rate, liver function biochemical index, pathological changes in liver tissues, ultrastructural changes, and biliary component change were observed by the methods of biochemical and pathological technology. Results①The gallstone formation rate of group B and C were obviously higher than group A (P＜0.05 or P＜0.01). The gallstone formation rate of D and E were obviously lower than group C (P＜0.05 or P＜0.01). ②There were abnormal ultrastructural changes to certain extent in models B and C. Compared with the model groups, the D and E groups improved the ultrastructural changes and pathological changes in liver tissues, especially D group. ③The level of AST，ALT，r-GT and indirect bilirubin in serum of groups D