太子参醇提物对大鼠组织及红细胞抗氧化活性.docVIP

太子参醇提物对大鼠组织及红细胞抗氧化活性 　 作者：　张振明，葛斌，许爱霞，袁逸铭，高湘 【关键词】 太子参醇提物 Antioxidative activity of EPHPH on tissues and erythrocytes of rats 　　【Abstract】 AIM: To study the antioxidative activity of the alcoholic extract from Pseudostellaria heterophylla Pax et Hoffm (EPHPH) and its mechanism. METHODS: The peroxidation in homogenates of heart and liver and kidney of rats was induced by #12539;HO generation system Fe2++ascorbic acid. Malondialdehyde（MDA）was determined using TBA colorimetric method and superoxide（O2-）from zymosanstimulated neutrophils of rats was measured by NBT reduction method. H2O2caused hemolysis of erythrocytes was measured spectrometrically. RESULTS: MDA formation in homogenates of heart and liver and kidney of rats induced by #12539;HO generation system Fe2++ascorbic acid was inhibited differently by 6.7, 13.4, 26.8, 53.5, 107.0 and 214.0 μg/L EPHPH, whose IC50 that inhibited MDA formation in heart, liver and kidney was 57.0, 51.5 and 53.2 μg/L respectively, whose relation between quantity and efficiency showed all negative and whose relative coefficient was -0.891,-0.883 and -0.893 respectively (all Plt;0.001). O2- generation from zymosanstimulated neutrophils of rats was inhibited distinctly by EPHPH, whose IC50 that inhibited O2- generation was 83.1 μg/L, whose relation between quantity and efficiency showed negative and whose relative coefficient was -0.894(Plt;0.001). H2O2caused hemolysis of erythrocytes was inhibited distinctly by EPHPH whose IC50 that inhibited hemolysis of erythrocytes was 86.5 μg/L, whose relation between quantity and efficiency showed negative and whose relative coefficient was -0.901 (Plt;0.001). CONCLUSION: EPHPH exerts its effect on antioxidation by scavenging #12539;HO, O2- and H2O2 in the biological system. 　　【Keywords】 EPHPH; free radicals; hemolysis extent; erythrocytes; activitiy of antioxidation 　　【摘要】 目的： 研究太子参醇提物的抗氧化活性及其机制. 方法： 用#12539;HO生成系统Fe2++抗坏血酸诱导大鼠心、肝、肾组织匀浆脂质过氧化，TBA比色法测定MDA含量；NBT还原法测酵母多糖A刺激大鼠中性粒细胞产生的O2-；分光光度法测H2O2诱