小儿哮喘花剥苔产生免疫机制探究.docVIP

小儿哮喘花剥苔产生免疫机制探究 　 作者：李福凤，李果刚，吴友忠，李靖，章晓鹰，王惠芳，王忆勤 【关键词】 花剥苔; ［摘要］ 目的：从免疫学角度探讨哮喘患儿花剥苔形成的机制。方法：采用免疫组化方法检测舌苔脱落上皮细胞中角蛋白13及bcl2蛋白的含量。应用流式细胞术检测外周血CD4+及细胞趋化因子受体3（cell chemokine receptor3, CCR3）的表达量。采用放射免疫法检测血清中皮质醇的含量。结果：哮喘花剥苔组患儿外周血CD4+和CCR3水平明显高于哮喘非花剥苔组及正常组（P＜0.05)；哮喘花剥苔组、非哮喘花剥苔组血清中皮质醇含量低于其余各组（P＜0.05)。哮喘花剥苔、非花剥苔组血清IgE水平明显高于其余各组（P＜0.05)。哮喘花剥苔组、非哮喘花剥苔组舌苔上皮脱落细胞中角蛋白13的含量明显低于哮喘非花剥苔组、非哮喘非花剥苔组和正常对照组（P＜0.05)。哮喘花剥苔组患儿舌苔上皮脱落细胞中bcl2蛋白含量与其余各组比较无统计学差异。结论：花剥苔的形成与免疫反应密切相关，血清皮质醇水平降低、CD4+及CCR3增多，可能促进了花剥苔的形成。同时，舌上皮细胞角化障碍及舌上皮细胞凋亡亦可能促进花剥苔的形成。 　　［关键词］ 花剥苔; 哮喘; 免疫 　　Immunological mechanism of exfoliative tongue fur in children with asthma 　　ABSTRACT Objective: To explore the immunological mechanism of exfoliative tongue fur in children with asthma. Methods: Thirtynine children with asthma, twentyeight children with repetitive respiratory tract infection (nonasthma) and eleven healthy children were divided into five groups, which were asthma with exfoliative fur or with nonexfoliative fur groups, nonasthma with exfoliative fur or with nonexfoliative fur groups and normal control group. The concentrations of keratin 13 and bcl2 in cells exfoliated from tongue fur were detected by immunohistochemical method. The expression levels of blood cell chemokine receptor3 (CCR3) and CD4+ were examined by flow cytometry, and the levels of serum cortisol and IgE were detected by radioimmunoassay. Results: The levels of blood CD4+ and CCR3 of children with asthma and exfoliative fur were higher than those in the asthma with nonexfoliative fur group and the normal control group (P＜0.05). The serum level of cortisol in the groups of asthma with exfoliative fur and nonasthma with exfoliative fur were lower than that in the other groups (Plt;0.05). The serum levels of IgE in asthma with exfoliative fur or with nonexfoliative fur groups were higher than that in the other groups (Plt;0.05). Concentrations of keratin 13 in the cells exfoliated from tongue fur in the groups of asthma or nonasthma with exfoliative fur