新城疫病毒合用中药黄芪诱生内源性干扰素探究.doc

新城疫病毒合用中药黄芪诱生内源性干扰素探究 　中国论文联盟编辑。 【摘要】 目的 研究 新城疫病毒（NDV）合用中药黄芪在小鼠体内诱生内源性干扰素-α及对小鼠的免疫调节作用。 方法 实验分3部分，实验1：分别以NDV、黄芪及二者合用予小鼠口腔喷洒给药，连续10日后以ELISA法测定小鼠唾液中干扰素-α和SIgA的含量；实验2：分别以NDV、黄芪及二者合用予小鼠腹腔注射给药（黄芪灌胃方式给药），ELISA法测定给药10日后血清干扰素-α的含量；实验3：分为NDV、黄芪及二者合用的高、中、低三种剂量，给药方法同第二组，6天后MTT法测定脾脏NK细胞的活性。每组分别设置阴性对照。结果 NDV和黄芪口腔给药能升高小鼠唾液干扰素-α（35.54±10.20 IU/ml,5.69±1.35 IU/ml）和SIgA含量（68.88±15.47 ng/ml,44.32±9.87 ng/ml），腹腔给药能诱生血清干扰素-α（120.54±29.21 IU/ml,10.69±6.35 IU/ml），并能激活NK细胞活性(50.4％,32.1％)。NDV合用黄芪组的诱生效果远高于单独使用组（98.55±16.95IU/ml,101.31±27.59ng/ml,262.55±26.91IU/ml,75.6%），显示出较强的协同作用。结论 NDV合用黄芪能诱生小鼠内源性干扰素，并能发挥对小鼠SIgA和NK细胞的免疫调节作用。 毕业论文 【关键词】 新城疫病毒NDV 芪 内源性干扰素-α SIgA NK细胞 毕业论文 Abstract：Objective To study the induction of endogenous interferon-α(IFN-α) and immunological regulation of Newcastle Disease Virus(NDV)and Milkvetch Root(MR)combinating administrated in mice.Methods The re were 3 parts.Part 1:the IFN-α and SIgA concentration in saliva were detected by ELISA after NDV and MR were sprinkled in mouse oral cavity for 10 days.Part 2:the level of IFN-α in serum were detected but the drug were intraperitoneal administrated.Part 3:the activity of NK cell in spleen were measured by MTT method after the same treatment as group 2 for 6 days.Results The concentration of IFN-α(35.54±10.20 IU/ml,5.69±1.35 IU/ml)and content of SIgA（68.88±15.47 ng/ml,44.32±9.87 ng/ml）in saliva had been promoted after NDV and MR administrated in oral cavity,and the serum level of IFN-α（120.54±29.21 IU/ml,10.69±6.35 IU/ml）had been induced and spleen NK cell(50.4％,32.1％)had been activated after it administrated in abdominal cavity.When NDV and MR combinating administrated,they synergistically enhanced the production of endogenous IFN-α and stimulated the immunological regulation, the detected data(98.55±16.95 IU/ml,101.31±27.59 ng/m,262.55±26.91 IU/ml,75.6%)were more higher than they were separated used(P＜0.05).Conclusion NDV combinated with MR used in mice showed a strong IFN-α inducing capacity,and then it c