明适应过程中SD大鼠视锥细胞反应变化规律.doc

明适应过程中SD大鼠视锥细胞反应变化规律 　 ; 作者：顾永昊 张作明 侯豹可 郭群 李莉 龙潭 【关键词】; 视锥细胞　　Properties of rat cone response during light adaptation　　【Abstract】; AIM:; To investigate the properties of rat cone response during light adaptation. METHODS:; Thirty rats were divided into 3 groups. Their 0.5 Hz and 20 Hz cone response was recorded respectively in adapted dark and 10 s, 5, 10, 15, 20, 25 and 30 min after background light was turned on. The stimulus intensities for the three group were 1.1, 3.5 and 11.0 cd#12539;s#12539;m-2, respectively. RESULTS:; There were some small oscillatory waves overlapped on the 0.5 Hz response bwave. For the 0.5 Hz response, with light adaptation, all three intensities’ implicit time were shortened after the lighton, shortest at about 10 min, and prolonged again after then. The amplitudes also decreased after the lighton. However, with high intensity, the amplitude began to increased at about 5 min, reached an asymptotic state at 10-15 min but didn’t reach the dark adapted level. For the 20 Hz response, the low intensity’s implicit time delayed during light adaptation while that of the high intensity’s shortened in the whole course. The low intensity’s amplitude decreased after the lighton while that of high intensity’ increased and reached an asymptotic state at 10-15 min, which was larger than that in dark adapted level. CONCLUSION:; The stimulus frequency of 20 Hz, with the intensity of at least 3.5 cd#12539;s#12539;m-2 and the duration for light adaptation more than 10 minutes, is proved better in recording the rat cone response. The changes of rat cone response during the light adaptation may reflect the summary effects of rods, cones and more neuromodulatary cells.　　【Keywords】 cone; light adaptation; rats　　【摘要】; 目的：探索明适应过程中SD大鼠视锥反应的变化规律. 方法：30只SD大鼠分为3组，分别在暗适应条件下、背景光打开后10 s和5，10，15，20，25及30 min，使用1.1, 3.5和11.0 cd#12539;s#12539;m-2的刺激光强，记录0.5 Hz及20 Hz反应. 结果：高光强时，0.5 Hz反应b波上叠加有子波. 明适应开始后，0.5 Hz反应b波的潜伏期开始缩短，在10 min左右达到最低，之后又开始延长；幅值也出现下降，低光强（1.1 cd#12539;s#