核干细胞因子基因在胃癌、结肠癌及直肠癌组织中表达.docVIP

核干细胞因子基因在胃癌、结肠癌及直肠癌组织中表达 　 作者：王胜，袁志诚，马珪，许文荣 【摘要】 　　　目的： 建立并采用实时定量PCR法检测核干细胞因子（nucleostamin，NS)基因在胃癌、结肠癌和直肠癌组织中的表达，研究该基因在胃肠道癌症诊断中的意义。方法： 构建NS基因和内参基因GAPDH标准品，并采用RTPCR和实时定量 PCR 检测43例胃癌、结肠癌和直肠癌患者肿瘤组织NS基因的表达。结果： RTPCR结果显示NS基因在胃癌组织中的阳性率为90%（27/30），在直肠癌组织中的阳性率为83.3%（5/6），在结肠癌组织中的阳性率为100%（7/7）。NS基因在胃肠道肿瘤组织中表达上调，表达水平与病理分期未见显著相关性，但表达量与癌组织分化程度显著相关，分化程度较低的癌组织NS表达较高，分化程度较高的癌组织NS表达较低(P＜0.05)。结论： NS基因过表达可能在胃肠道肿瘤的发生中起着一定的作用，该基因可能会成为胃肠道肿瘤治疗的靶基因之一，检测该基因的表达有助于胃肠道肿瘤的诊断。 【关键词】 核干细胞因子； 实时定量 PCR； 胃癌； 结肠癌； 直肠癌 　　［Abstract］Objective: To establish a method of realtime PCR to detect expression of nucleostamin(NS) mRNA, and to analyze its significance in diagnosing alimentary canal cancer.Methods： RTPCR and realtime quantitative PCR were used to investigate the expression of NS in cancer tissues of alimentary canal cancer(43 cases). Results： The results of RTPCR showed that NS was positive in cancer tissues 90%(27/30) of the patients with gastric cancer, 83.3%(5/6) of the patients with carcinoma of rectum and 100%(7/7) of the patients with colon carcinoma. The results of realtime quantitative PCR showed that NS was up regulated in the tissues of gastric cancer , rectum carcinoma and colon carcinoma. The correlation between the expression of NS in cancer tissue and development stage of alimentary canal cancer could not be found, but the expression of NS in cancer tissue was correlated to the differentiation stage of cancer tissue and prognosis of the patients. NS was expressed at a higher level in the earlier stage of differentiation than that in the advanced stage(P＜0.05). Conclusion： NS gene may play a role in tumorigenesis and be a novel maker for alimentary canal cancer. 　　［Key words］nucleostamin; realtime quantitative PCR; gastric cancer; colon carcinoma; rectal carcinoma 　　人类核干细胞因子（nucleostemin, NS）基因位于3p21.1, 属于原癌基因，其cDNA全长1 650 bp, 编码一个由549 个氨基酸残基组成的相对分子质量为61.8 ku的蛋白质，对干细胞的增殖和分化有非常重要的意义。该基因的高表达可以阻止干细胞分化，导致永生化［1-4］，从而导致肿瘤的发生。对NS基因在消化道肿瘤组织中表达的研究国内文献报道较少，因此，本研究检测了N