皂角刺皂苷对大鼠前列腺增生模型影响实验探究.docVIP

皂角刺皂苷对大鼠前列腺增生模型影响实验探究 　【摘要】 　　目的探讨中药皂角刺提取物皂角刺皂苷对前列腺增生(BPH)的影响及作用机制。方法将40只SD大鼠去势7d后皮下注射丙酸睾酮5 mg/kg，同时实验组灌胃给予皂角刺皂苷溶液4，2 mg/kg，癃闭舒组灌胃给予癃闭舒胶囊100 mg/kg。于给药30 d后处死，观察前列腺重量和组织结构改变，并通过免疫组化法检测PCNA和bFGF表达的变化。结果实验组大鼠前列腺重量、PCNA指数及bFGF表达水平均低于模型对照组，其中尤以皂角刺皂苷大剂量组最显著(Plt;0.01)。皂角刺皂苷大剂量组与癃闭舒组比较差异亦有显著性，而皂角刺皂苷小剂量组与癃闭舒组比较无显著差异。结论皂角刺能明显抑制模型大鼠的BPH，其机制可能是通过抑制前列腺细胞的增殖及减少前列腺组织中bFGF的表达而实现的。 【关键词】 皂角刺 皂角刺皂苷 前列腺增生 　　Abstract：ObjectiveTo study the effect of Chinese Honeylocust Spine on benign prostatic hyperplasia(BPH) and its mechanism.Methods Forty ovariectomized SD rats were administered testosterone propionate 5mg/kg subcutaneously. At the same time, saponin high and low dose groups were treated respectively with saponin fluid 4mg/kg and 2mg/kg by oral administration and Longbishu Capsule(LBS) group with LBS 100mg/kg. The rats were decapitated after 30 days, the weight of prostate and lightmicroscopic examination were undertaken, PCNA index and expression of bFGF were analyzed by means of immunochemical technology. ResultsThe weight of prostate, PCNA index and the content of bFGF in three medicated groups were significantly less than those of the model group, especially in saponin high dose group. The difference between saponin high dose group and LBS group was also remarkable. But it was not between saponin low dose and LBS group. ConclusionSaponin could suppress the benign prostatic hyperplasia. The mechanism might be the inhibition of cell hyperplasia of prostate and the expression of bFGF. 　　Key words：Chinese Honeylocust Spine; Benign prostatic hyperplasia; Experimental study 　　中药皂角刺为植物皂角树的棘，是一味传统的中药材，具有穿透肿毒、破坚消积、消肿排脓等功效。现代研究表明皂角刺含有皂苷、黄酮、酚类、氨基酸等多种生物活性成分，具有抗凝、免疫调节、抗肝纤维化等作用。祖国医学史上很早就有用皂角刺治疗癃闭的记载，现代中医运用皂角刺治疗前列腺增生亦取得了较好的疗效。但对皂角刺治疗前列腺增生的主要活性成分及可能作用机制还未见研究，我们拟通过研究皂角刺皂苷对前列腺增生模型的作用，探讨皂角刺治疗前列腺增生的主要活性成分及可能机制。 　　1 材料与方法 　　1.1 材料 　　丙酸睾酮注射液，2 ml/支，含丙酸睾酮25 mg/ml，由天津氨基酸公司生产(批号050419)；皂角刺皂苷由本实验室提取。癃闭舒胶囊，0.3 g/粒，石家庄科迪药业有限公司生产(批号050401); PCNA单克隆抗体及LSAB试剂盒由Dako公司提供；bFGF抗体由美国Sigma公司生产；分析天平；双目光学显微镜；德国Lei