益肾平喘活血合剂对哮喘气道重塑大鼠血清及气道上皮炎性因子影.docVIP

益肾平喘活血合剂对哮喘气道重塑大鼠血清及气道上皮炎性因子影 　 作者：张燕萍，张元元，苗青，王书臣，谭素贞 【摘要】 目的初步探讨益肾平喘活血合剂对哮喘气道重塑大鼠血清及气道上皮炎性因子的影响。方法SD大鼠60只，随机分为正常组，造模组，激素组和中药高、中、低剂量组各10只。按文献方法制成支气管哮喘模型。用放免法测定血清IL-2、IL-4浓度，免疫组化法测定大鼠气道TGF-β，苦味酸天狼星红染色，观察气道胶原分布和变化。结果①与正常组比较，中药3个组血清IL-2水平下降，IL-4水平升高均轻于造模组和激素组，与造模组比较有显著差异（Plt;0.01），与激素组比较无显著差异（Pgt;0.05）；中药组组间比较，高、中剂量组优于低剂量组（Plt;0.05）。②TGF-β免疫组化造模组阳性反应最强，呈深棕色，激素组，中药3个组阳性反应稍弱，呈棕黄色至浅黄色，正常组呈淡浅黄色。③苦味酸天狼星红染色，偏振光显微镜观察，造模组支气管粘膜及上皮下Ⅰ型和Ⅲ型胶原纤维明显增多；激素组较造模组减少，中药低剂量组改变与激素组类似，中药中、高剂量组胶原纤维沉积少于激素组；正常组可见少量胶原纤维分布。结论益肾平喘活血合剂治疗哮喘慢性持续期的作用途径，可能有一部分是通过调节IL-2、IL-4、TGF-β水平，减少气道壁胶原等来延缓支气管哮喘气道重塑的形成。 【关键词】 支气管哮喘； 气道重塑； 炎性因子 ChinaAbstract：ObjectiveTo investigate the effect of YiShenHuoXue decoction on serum inflammation factor of bronchus asthma airway remolding model.MethodsSixty SD rats were randomly divided into six groups: blank group,asthma group,hormone group, and high, middle and low dosage Chinese medicine groups, 10 rats in each group.The asthmatic model was established by sensitization and challenge with ovalbumin.Radioimmunoprecipitation was used to determine the contents of serum IL-2, IL-4. ELISA was used to measure the level of serum TGF-β. Picric acid sirius red dyeing was used to observe the dostritmtion and change of collagen in the rat airway. Results(1) Compared with the blank group, serum IL-2 was descreased, and IL-4 was increased in other five groups. The change of rat’s serum in the three Chinese medicine groups was smaller than in the asthma group and hormone group. The levels of serum IL-2, IL-4 among Chinese medicine groups and the asthma group were different. （Plt;0.01）. They were similar among the Chinese medicine groups and hormone group （Pgt;0.05）The high and middle dosage groups were better than the low dosage group （Plt;0.05）.(2) The positive reaction of serum TGF-β in the asthma group was the strongest, deep brown look. The positive reaction of serum TGF-β in the hormone group and the Chinese medicine were much lighter, brown and buff look.The blank group looked lik