细胞外信号调节激酶1-2阻断剂PD98059对小鼠淋巴细胞增殖影响.docVIP

细胞外信号调节激酶1/2阻断剂PD98059对小鼠淋巴细胞增殖影响 　 ; 作者：滕振平, 邢飞跃, 王通, 李莉平 毕业论文 【关键词】; 细胞外信号调节激酶1/2;,淋巴细胞;,增殖;,细胞周期 毕业论文 　　Effect of extracellularsignal regulated kinase 1/2 specific repressor PD98059 on murine lymphocyte proliferation 毕业论文 　　[Abstract]; AIM: To investigate the effect of PD98059, a specific repressor of extracellular signal regulated kinase1/2 (ERK1/2), on the proliferation of the murine lymphocytes. ; METHODS: Lymphocytes were isolated from lymphoid nodes of BALB/c mouse. After stained with carboxy fluorescein diacetate, succinimidyl ester(CFDASE), the cells were stimulated with polyclonal activators, concanavalin(ConA) and phorbol 12,13dibutyrate(PDB) plus ionomycin(Ion). The proliferation and cellcycle of lymphocytes were analyzed using flow cytometry（FCM）. RESULTS: CFDASE staining showed that 5, 10, 20, 30 and 40 μmol/L PD98059 could significantly inhibit the proliferation of murine lymphocytes induced by ConA. The inhibitory effect was dosedependent(r=0.985, Plt;0.01). The optimal concentration of 30 μmol/L PD98059 was selected and its inhibitory effect was significantly decreased with prolongation of time (Plt;0.01); but the inhibitory rate increased. Analysis of the cell cycle induced by ConA revealed that the cells was blocked into S and G2/M phases as the concentration of PD98059 increased. However, the cell number in subG0/G1 peak (apoptotic peak) had no significant change under the concentration range used. PD98059 had the similar effect on the cells treated with PDB plus Ion, but S and G2/M phases were more distinct. CONCLUSION: PD98059 can inhibit the proliferation of the murine lymphocytes and blocked them into S and G2/M phases, indicating the activation of ERK1/2 signaling pathway plays an important role in the proliferation of lymphocytes. 毕业论文 　　[Keywords]extracellularsignal regulated kinase1/2; lymphocytes;proliferation; cell cycle 毕业论文 　　[摘 要]; 目的: 探讨细胞外信号调节激酶1/2（ERK1/2）信号通路的特异性阻断剂PD98059对小鼠淋巴细胞体外增殖的影响。方法: 分离小鼠淋巴结细胞, 藉羧基荧光素乙酰乙