肺纤方颗粒对盐酸平阳霉素致肺间质纤维化大鼠血清转化生长因子β.docVIP

肺纤方颗粒对盐酸平阳霉素致肺间质纤维化大鼠血清转化生长因子β 　 作者：张伟, 姜良铎, 张晓梅, 吴建军, 邓媛, 鲁香凤 【摘要】 观察肺纤方颗粒抗大鼠肺间质纤维化的作用，及其对大鼠血清转化生长因子β1（transforming growth factorβ1, TGFβ1）和血小板衍生生长因子（plateletderived growth factor，PDGF）的影响。方法：72只雄性Wistar大鼠经气管插管灌注盐酸平阳霉素1 mg/kg复制大鼠肺间质纤维化模型，随机分入模型组、泼尼松组和肺纤方组，每组24只；15只雄性Wistar大鼠经气管插管灌注等体积生理盐水作为假手术组。造模后24 h开始常规灌胃给药，泼尼松组给予醋酸泼尼松5 mg/kg，肺纤方组给予肺纤方颗粒1.25 g/kg，模型组和假手术组给予等体积生理盐水，1次/d。分别于第14、28、45 天取材，假手术组每批5只，其余各组每批8只。检测其肺组织病理改变及血清中TGFβ1和PDGF的水平。结果：与模型组和泼尼松组比较，在第45天时病理检测显示肺纤方组肺间质纤维化的程度较轻；血清TGFβ1和PDGF检测结果显示，模型组大鼠两种因子水平升高，第45天时升高最明显，并高于假手术组（Plt;0.05）。肺纤方组与泼尼松组的TGFβ1变化与模型组相似（Pgt;0.05），两组间TGFβ1变化比较差异无统计学意义（Pgt;0.05）。肺纤方组PDGF水平在第14天时与其余各组比较显著升高（Plt;0.01），然后持续下降，至第45天与假手术组接近（P=0.792）；而模型组和泼尼松组PDGF水平皆随时间延长逐渐上升，第45天时均显著高于假手术组（Plt;0.01）。结论：肺纤方有一定的减轻盐酸平阳霉素致大鼠肺间质纤维化的作用，其作用机制可能与对血清PDGF水平的调节作用有关。 【关键词】 肺纤方颗粒; 肺疾病, 间质性; 肺纤维化; 转化生长因子β1; 血小板衍生生长因子; 大鼠 　　Objective: To observe the effects of Feixian Formula, a compound traditional Chinese herbal medicine for treating pulmonary fibrosis, on bleomycininduced pulmonary fibrosis in rats, and its influence on serum transforming growth factorβ1 (TGFβ1) and plateletderived growth factor (PDGF). Methods: Seventytwo male Wistar rats were infused with bleomycin (1 mg/kg) through tracheal intubation to induce pulmonary fibrosis, and they were randomly divided into untreated group (n=24), prednisonetreated group (n=24) and Feixian Formulatreated group (n=24). Fifteen male Wistar rats of the shamoperated group were infused with equivalent normal saline. Twentyfour hours after operation, prednisone (5 mg/kg) and Feixian Formula (1.25 g/kg) were given to the prednisonetreated group and Feixian Formulatreated group respectively by intragastric administration once a day. Equivalent saline was administered to rats of the untreated group and shamoperated group. On the 14th, 28th and 45th day, 5 rats in the shamoperated group and 8 rats in each of the other three groups were dissected to observe pathologic changes of the lung tissues, and the