胆囊切除术对豚鼠胃肠移行性复合波及胆酸池大小影响.docVIP

胆囊切除术对豚鼠胃肠移行性复合波及胆酸池大小影响 　 作者：张雪梅, 董蕾, 刘丽娜, 畅碧侠，何谦，李谦 【关键词】 胆囊切除术 Effects of cholecystectomy on gastrointestinal myoelectric activity and bile acid pool size in guinea pigs 　　【Abstract】 AIM: To investigate whether the bile acid pool size after cholecystectomy is correlated to the gastrointestinal migrating myoelectric complex (MMC) in guinea pigs. METHODS: Gallbladder motilities were assessed before cholecystectomy and interdigestive gastrointestinal motilities were continuously monitored using bipolar electrodes in conscious guinea pigs before and 4 weeks after surgery in standard diet group and high cholesterol diet (cholesterol gallstone) group. Total bile acid pool sizes were measured by isotope dilution method. RESULTS: After cholecystectomy, there were parallel falls in duration of phase I, II, III and MMC cycle duration but increase in amplitude in the guinea pigs with normal gallbladder function, and in the guinea pigs with cholesterol stones, but no significant difference was found (Pgt;0.05). The bile acid pool was definitely small in the gallbladder stone (GS) guinea pigs compared with that in normal guinea pigs (Plt;0.05) and became slightly smaller after cholecystectomy. The bile acid in gallbladder bile and fecal bile acid slightly increased in GS guinea pigs after cholecystectomy, similar to those in guinea pigs with normal gallbladder function. CONCLUSION: Cholecystectomy produces a similar but not insignificant change in the bile acid pool size in guinea pigs with normal gallbladder function and in the guinea pigs with cholesterol stones. MMC is associated to the enterohepatic circulation of bile acids. Gastrointestinal dyskinesia is not the result postcholecystectomy syndrome. 　　【Keywords】 cholecystectomy; enterohepatic circulation of cholic acid; cholic acid pool; migrating motor complex; gallbladder motility 　　【摘要】 目的： 研究豚鼠胆囊切除术后胃肠移行性复合波(MMC)是否与胆酸池大小有关. 方法： 60只豚鼠分别给予正常饮食8 wk（A组、B组），10 g/L胆固醇饮食8 wk（C组、D组），喂养结束后，评估胆囊动力并将电极植入胃肠道；其中B组、D组同时行胆囊切除术，术后