脐血胰岛素样生长因子2及印记基因H19及胎儿生长受限关系.docVIP

脐血胰岛素样生长因子2及印记基因H19及胎儿生长受限关系 　 作者：杨新园，苟文丽，王海燕 【摘要】 目的 探讨脐血胰岛素样生长因子2(IGF2)和H19印记状态与胎儿生长受限(FGR)的关系。 方法 采用放射免疫法测定42例FGR孕妇( 研究 组)和晚期正常妊娠妇女30例(对照组)脐血中IGF2水平，同时采用逆转录多聚酶链反应(RTPCR)技术，检测胎盘组织中H19基因印记状态。结果 ①研究组脐血IGF2水平为(1.52±0.20)μg/L，明显低于对照组的(1.97±0.21)μg/L(Plt;0.05)。②研究组中杂合子20例，其中9例双等位基因表达；对照组中杂合子14例，均为单等位基因表达；研究组H19基因印记丢失明显高于对照组(Plt;0.05)。③研究组H19双等位基因表达的病例脐血IGF2水平均明显低于H19杂合性丢失和H19单等位基因表达病例(Plt;0.05)；H19杂合性丢失和H19单等位基因表达病例脐血IGF2水平无显著性差异。结论 妊娠晚期IGF2减低是导致FGR发生的原因之一；H19基因印记丢失下调IGF2基因间接 影响 胎儿生长发育。 论文代写 【关键词】 胰岛素样生长因子2；印记基因H19；胎儿生长受限 　　Study on relationship between fetal growth retardation and expression　of insulinlike growth factor2 and H19 imprinted genes 论文代写 　　ABSTRACT: Objective To study the relationship between fetal growth retardation (FGR) and expression of insulinlike growth factor2 and H19 imprinted genes. Methods Immunoradioassay was used to detect the insulinlike growth factor2(IGF2)level in cord blood from 42 pregnant women with FGR (FGR group) and 30 normal pregnant women (normal group). Reverse transcriptionpolymerase chain reaction method (RTPCR) was used to detect H19 imprinted genes allelespecific expression level in placenta. Results The level of IGF2 in cord blood from FGR group was (1.52±0.20)μg/L. It was significantly decreased as compared with that from the normal group (1.97±0.21)μg/L (Plt;0.05). Among the 42 cases of FGR tissue samples, we observed 20 heterozygous cases. In normal tissues there were 14 heterozygous cases. Nine cases of 20 in the FGR group showed biallelic expression of H19, whereas no biallelic expression was demonstrated in the normal group (Plt;0.05). The level of IGF2 from biallelic expression of H19 in FGR group was low as compared with that from samples of loss of heterozygosity and loss of biallelic expression cases. Conclusion The decrease of fetal serum concentration of IGF2 is one of the direct factors contributing to FGR. And loss of imprinting of H19 gene may be one of the indirect factors contrib