草鱼线粒体型超氧化物歧化酶的生化遗传特性3颜勤罗琛.PDF

动物学报 　50 (3) :389 - 394 , 2004 A cta Zoologica S inica 　　　　　 　 Genetic and biochemical characteristics of mitochondrial superox ide dismutase in grass carp Ctenopharyngodon idellus YAN Qin , L UO Chen Institute of Biology , Hunan Normal University , Changsha 　410081 , China Abstract 　Superoxide dismutase is an evolutionarily conserved enzyme with an important role in protecting organisms and cells from the damages caused by oxygen free radicals. As a molecular clock and genetic marker , superoxide dismutase has been widely used in evolutionary studies , population genetics , and identification of different strains in the same species. However , the biochemical and genetic features of the mitochondrial superoxide dismutase have not been well characterized in fishes. In this study , we investigated the genetic phenot ypes and biochemical features of grass carp Ctenopharyn godon idellus mitochondrial superoxide dismutase (fmSOD) with polyacrylamide gradient gel electrophore sis. Our data revealed three isoforms of fmSOD which were named fmSOD 1 , fmSOD 2 and fmSOD 3 according to their positions from the positive pole to the negative pole. The combination of three isoforms of fmSOD constitute three distinct biochemical phenot ypes. Phenotype 1 was only associated with the fastest migratory isoform fmSOD 1 and phe notype 3 with the slowest migratory isoform fmSOD 3 , while phenotype 2 was associated with all three isoforms. In the wild group of grass carp , all three phenotypes were observed , whereas in the mitogynogenetic group , only phenot ypes 1 and 3 were observed. The ratio of the three phenotypes in the wild group was consist