茶多酚对人γδT淋巴细胞及结肠癌细胞株SW480影响.doc

茶多酚对人γδT淋巴细胞及结肠癌细胞株SW480影响 　 作者：朱月蓉， 邱红， 刘军权， 陈复兴 【摘要】 目的： 观察茶多酚对人γδT细胞杀伤肿瘤细胞活性的影响和诱导人结肠癌细胞株（SW480）凋亡和死亡的作用。方法： 在体外用不同浓度的茶多酚诱导人γδT细胞和SW480细胞株，然后测定人γδT细胞杀瘤活性和对肿瘤细胞的作用。用不同浓度的茶多酚分别诱导SW480细胞4 h后，弃含有茶多酚的培养液再继续培养24 h，然后测定其死亡和凋亡数，用γδT细胞作对照组。结果： 茶多酚浓度350 mg/L，作用γδT细胞4 h后能明显增强γδT细胞的杀伤肿瘤细胞活性，与未诱导组和其他浓度组相比，Plt;0.01；γδT细胞对经茶多酚处理后的SW480细胞杀伤活性也明显高于对照组（Plt;0.01）。各种浓度的茶多酚分别与γδT和SW480细胞作用4 h，弃诱导液再继续培养24 h后，均能诱导其死亡和凋亡，在茶多酚浓度350 mg/L、诱导时间4 h时最明显；同一浓度的茶多酚诱导SW480细胞死亡和凋亡率明显高于γδT细胞组。结论： 茶多酚在一定浓度时能明显提高γδT细胞对肿瘤的杀伤活性；经茶多酚处理的SW480细胞更易被γδT细胞杀伤。茶多酚浓度在350 mg/L时能诱导SW480细胞凋亡而对人γδT细胞无影响。 【关键词】 茶多酚； γδ淋巴细胞； SW480细胞株； 抗肿瘤活性； 凋亡 　　［Abstract］ Objective: To observe cytotoxicity of γδT cell induced by tea polyphenols and the direct effects of tea polyphenols on human tumor cells SW480 cell strain death and apoptosis.Methods： γδT cells and SW480 cell strain were induced by tea polyphenols in vitro to examine cytotoxic activity of γδT cell and its direct effects on tumor cells. SW480 was induced by tea polyphenols continuously for 4 hours following being cultured without tea polyphenols for 24 hours, then the rate of death and apoptosis of SW480 were measured. γδT cell was used as control group. Results： Cytotoxicity of γδT cell induced by tea polyphenols with concentration of 350 mg/L was significantly higher than that of control group and other groups(Plt;0.01). Cytotoxicity of γδT cell on SW480 induced by tea polyphenols was higher than the control group(Plt;0.01).After being induced by tea polyphenols continuously for 1～8 hours following cultured without tea polyphenols for 24 hours, SW480 showed significant cell death and apoptosis in different concentration comparing with the control group. Tea polyphenols did not display significant effects on γδT cell. Conclusion： Tea polyphenols can significantly enhance cytotoxicity of γδT cell and the SW480 induced by tea polyphenols was easily killed by γδT cell. Tea polyphnols in certain concentration can induce cell death and apopto