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a new generation of pprig-based retroviral vectors新一代的pprig-based逆转录病毒载体
BMC Biotechnology BioMed Central
Methodology article Open Access
A new generation of pPRIG-based retroviral vectors
1 1 2
Olivier Albagli-Curiel* , Yann Lécluse , Philippe Pognonec ,
2 2
Kim E Boulukos and Patrick Martin*
Address: 1INSERM U790 and IFR54, Institut Gustave Roussy, PR1, 39 Rue Camille Desmoulins, 94805 Villejuif, France and 2CNRS UMR 6548,
Université de Nice, Parc Valrose, 06108 Nice, France
Email: Olivier Albagli-Curiel* - oalbagli@igr.fr; Yann Lécluse - ylecluse@igr.fr; Philippe Pognonec - POGNONEC@unice.fr;
Kim E Boulukos - BOULUKOS@unice.fr; Patrick Martin* - PMARTIN@unice.fr
* Corresponding authors
Published: 30 November 2007 Received: 13 September 2007
Accepted: 30 November 2007
BMC Biotechnology 2007, 7:85 doi:10.1186/1472-6750-7-85
This article is available from: /1472-6750/7/85
© 2007 Albagli-Curiel et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Retroviral vectors are valuable tools for gene transfer. Particularly convenient are
IRES-containing retroviral vectors expressing both the protein of interest and a marker protein
from a single bicistronic mRNA. This coupled expression increa
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