a powerful method for detecting differentially expressed genes from genechip arrays that does not require replicates一个强大的方法检测差异表达基因genechip数组不需要复制.pdfVIP

BMC Bioinformatics BioMed Central Research article Open Access A powerful method for detecting differentially expressed genes from GeneChip arrays that does not require replicates Anne-Mette K Hein* and Sylvia Richardson Address: Dept. of Epidemiology and Public Health, Imperial College London, Norfolk Place, London, UK Email: Anne-Mette K Hein* - a.hein@imperial.ac.uk; Sylvia Richardson - sylvia.richardson@imperial.ac.uk * Corresponding author Published: 20 July 2006 Received: 02 March 2006 Accepted: 20 July 2006 BMC Bioinformatics 2006, 7:353 doi:10.1186/1471-2105-7-353 This article is available from: /1471-2105/7/353 © 2006 Hein and Richardson; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Studies of differential expression that use Affymetrix GeneChip arrays are often carried out with a limited number of replicates. Reasons for this include financial considerations and limits on the available amount of RNA for sample preparation. In addition, failed hybridizations are not uncommon leading to a further reduction in the number of replicates available for analysis. Most existing methods for studying differential expression rely on the availability of replicates and the demand for alternative methods that require few or no re