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a semi-quantitative assay of overall dna methylation status using methyl-cpg binding protein (mbd1)整体的半定量测定dna甲基化状态使用methyl-cpg结合蛋白(mbd1)
Zhang et al. BMC Research Notes 2012, 5:234
/1756-0500/5/234
RESEARCH ARTICLE Open Access
A semi-quantitative assay of overall DNA
methylation status using Methyl-CpG binding
protein (MBD1)
*
Chunxiao Zhang, Runsheng Li and Ruqian Zhao
Abstract
Background: In mammals, DNA methylation at the 5-position of cytosine is the most essential epigenetic
modification. Changes in the level of genome-wide DNA methylation (also known as overall DNA methylation) are
associated with alterations in gene expression, thereby contributing to the phenotypic and physiological diversity.
Current technologies for detecting overall DNA methylation either suffer from low sensitivity or require
sophisticated equipment. Studies on domestic animals are hampered by the lack of complete and annotated
genomic information.
Results: Here we report a rapid slot blot method using methyl-CpG binding protein (MBD1) to exam the level of
overall DNA methylation in pigs and chickens. Using this rapid approach, we determined the methylation status in
various DNA samples of a Chinese indigenous (Erhualian) and a Western (Large White) breed of pigs. We also chose
day 18 embryos (E18) and newly hatched chicks (D1) of a Chinese indigenous chicken breed (Wen’s yellow-
feathered broiler chicken) for genome-wide DNA methylation analysis. The results revealed tissue- and breed-
specific differences, as well as age-dependent variations, in the level of overall DNA methylation.
Conclusion: The results showed that the slot blot assay is a sensitive, highly specific and convenient method for
semi-quantitative estimation of overall DNA methylation with no species specificity. This method does not require
sophisticated equipment, such as high performance liquid chromatography (HPLC), or expensive technolog
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