a simple technique for quantifying apoptosis in 96-well plates一个简单的技术来量化细胞凋亡在96 -孔板.pdfVIP

BMC Biotechnology BioMed Central Methodology article Open Access A simple technique for quantifying apoptosis in 96-well plates Deborah Ribble, Nathaniel B Goldstein, David A Norris and Yiqun G Shellman* Address: UCHSC at Fitzsimons, Dermatology Department, Mail Stop #8127, PO Box 6511, Aurora, CO 80045, USA Email: Deborah Ribble - Deborah.Ribble@; Nathaniel B Goldstein - Nathan.Goldstein@; David A Norris - David.Norris@; Yiqun G Shellman* - Yiqun.Shellman@ * Corresponding author Published: 10 May 2005 Received: 24 January 2005 Accepted: 10 May 2005 BMC Biotechnology 2005, 5:12 doi:10.1186/1472-6750-5-12 This article is available from: /1472-6750/5/12 © 2005 Ribble et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Analyzing apoptosis has been an integral component of many biological studies. However, currently available methods for quantifying apoptosis have various limitations including multiple, sometimes cell-damaging steps, the inability to quantify live, necrotic and apoptotic cells at the same time, and non-specific detection (i.e. false positive). To overcome the shortcomings of current methods that quantify apoptosis in vitro and to take advantage of the 96-well plate format, we present here a modified ethidium bromide and acr