a strategy to setup codominant microsatellite analysis for high-resolution-melting-curve-analysis (hrm)策略设置共显性的微卫星分析high-resolution-melting-curve-analysis(人力资源管理).pdfVIP

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a strategy to setup codominant microsatellite analysis for high-resolution-melting-curve-analysis (hrm)策略设置共显性的微卫星分析high-resolution-melting-curve-analysis(人力资源管理).pdf

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a strategy to setup codominant microsatellite analysis for high-resolution-melting-curve-analysis (hrm)策略设置共显性的微卫星分析high-resolution-melting-curve-analysis(人力资源管理)

BMC Genetics BioMed Central Methodology article Open Access A Strategy to Setup Codominant Microsatellite Analysis for High-Resolution-Melting-Curve-Analysis (HRM) Eduard Mader*, Brigitte Lukas and Johannes Novak Address: Institute for Applied Botany and Pharmacognosy, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria Email: Eduard Mader* - eduard.mader@vu-wien.ac.at; Brigitte Lukas - brigitte.lukas@vu-wien.ac.at; Johannes Novak - johannes.novak@vu- wien.ac.at * Corresponding author Published: 3 November 2008 Received: 3 July 2008 Accepted: 3 November 2008 BMC Genetics 2008, 9:69 doi:10.1186/1471-2156-9-69 This article is available from: /1471-2156/9/69 © 2008 Mader et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: High resolution melting curve analysis (HRM) is a technique that measures exactly the decreasing fluorescence of intercalating dye in the process of dissociation of double stranded DNA. The measurement is immediately following PCR in a one-step, closed-tube method. The shape of the melting curve depends on the GC content, length and sequence of the amplicon. Hence it is a powerful, fast and cheap method to detect Single Nucleotide Polymorphisms (SNPs) and other mutations. Results