a sweetpotato gene index established by de novo assembly of pyrosequencing and sanger sequences and mining for gene-based microsatellite markers甘薯基因索引建立的新创的焦磷酸测序和桑格序列和矿业为基于基因微卫星标记.pdfVIP

下载本文档

22
0
约6.28万字
约 10页
2017-09-11 发布于上海
举报
版权申诉

a sweetpotato gene index established by de novo assembly of pyrosequencing and sanger sequences and mining for gene-based microsatellite markers甘薯基因索引建立的新创的焦磷酸测序和桑格序列和矿业为基于基因微卫星标记.pdf

1、本文档共10页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

a sweetpotato gene index established by de novo assembly of pyrosequencing and sanger sequences and mining for gene-based microsatellite markers甘薯基因索引建立的新创的焦磷酸测序和桑格序列和矿业为基于基因微卫星标记

Schafleitner et al. BMC Genomics 2010, 11:604 /1471-2164/11/604 RESEARCH ARTICLE Open Access A sweetpotato gene index established by de novo assembly of pyrosequencing and Sanger sequences and mining for gene-based microsatellite markers 1* 1 2 3 3 3 Roland Schafleitner , Luz R Tincopa , Omar Palomino , Genoveva Rossel , Ronald F Robles , Rocio Alagon , 1 1 2 1 4 1 1 Carlos Rivera , Cynthia Quispe , Luis Rojas , Jaime A Pacheco , Julio Solis , Diogenes Cerna , Ji Young Kim , Jack Hou1, Reinhard Simon2 Abstract Background: Sweetpotato (Ipomoea batatas (L.) Lam.), a hexaploid outcrossing crop, is an important staple and food security crop in developing countries in Africa and Asia. The availability of genomic resources for sweetpotato is in striking contrast to its importance for human nutrition. Previously existing sequence data were restricted to around 22,000 expressed sequence tag (EST) sequences and ~ 1,500 GenBank sequences. We have used 454 pyrosequencing to augment the available gene sequence information to enhance functional genomics and marker design for this plant species. Results: Two quarter 454 pyrosequencing runs used two normalized cDNA collections from stems and leaves from drought-stressed sweetpotato clone Tanzania and yielded 524,209 reads, which were assembled together with 22,094 publically available expressed sequence tags into 31,685 sets of overlapping DNA segments and 34,733 unassembled sequences. Blastx comparisons with the UniRef100 database allowed annotation of 23,957 contigs and 15,342 singletons resulting in 24,657 putatively unique genes. Further, 27,119