activities of colistin- and minocycline-based combinations against extensive drug resistant acinetobacter baumannii isolates from intensive care unit patients粘菌素的活动u2014u2014和minocycline-based组合对广泛耐药鲍曼不动杆菌分离株从重症监护室的病人.pdfVIP

Liang et al. BMC Infectious Diseases 2011, 11:109 /1471-2334/11/109 RESEARCH ARTICLE Open Access Activities of colistin- and minocycline-based combinations against extensive drug resistant Acinetobacter baumannii isolates from intensive care unit patients 1 1 1 1 2 1* Wang Liang , Xiao-fang Liu , Jun Huang , De-mei Zhu , Jian Li and Jing Zhang Abstract Background: Extensive drug resistance of Acinetobacter baumannii is a serious problem in the clinical setting. It is therefore important to find active antibiotic combinations that could be effective in the treatment of infections caused by this problematic ‘superbug’. In this study, we analyzed the in vitro activities of three colistin-based combinations and a minocycline-based combination against clinically isolated extensive drug resistant Acinetobacter baumannii (XDR-AB) strains. Methods: Fourteen XDR-AB clinical isolates were collected. The clonotypes were determined by polymerase chain reaction-based fingerprinting. Susceptibility testing was carried out according to the standards of the Clinical and Laboratory Standards Institute. Activities of drug combinations were investigated against four selected strains and analyzed by mean survival time over 12 hours (MST12 h) in a time-kill study. Results: The time-kill studies indicated that the minimum inhibitory concentration (MIC) of colistin (0.5 or 0.25 μg/ mL) completely killed all strains at 2 to 4 hours, but 0.5×MIC colistin showed no bactericidal activity. Meropenem (8 μg/mL), minocycline (1 μg/mL) or rifampicin (0.06 μg/mL) did not show bactericidal activity. However, combinations of colistin at 0.5×MIC (0.25 or 0.125 μg/mL) with each of the above were synergistic and shown bactericidal activit