aminoadipate reductase gene a new fungal-specific gene for comparative evolutionary analysesaminoadipate还原酶基因的新fungal-specific基因比较进化分析.pdfVIP

BMC Evolutionary Biology BioMed Central BMC Evolutionary Biology x 2002, 2 Research article Aminoadipate reductase gene: a new fungal-specific gene for comparative evolutionary analyses Kwang-Deuk An, Hiromi Nishida*, Yoshiharu Miura and Akira Yokota Address: Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan E-mail: Kwang-Deuk An - aa17122@mail.ecc.u-tokyo.ac.jp; Hiromi Nishida* - hnishida@iam.u-tokyo.ac.jp; Yoshiharu Miura - ymiura@iam.u- tokyo.ac.jp; Akira Yokota - uayoko@mail.ecc.u-tokyo.ac.jp *Corresponding author Published: 3 April 2002 Received: 25 December 2001 Accepted: 3 April 2002 BMC Evolutionary Biology 2002, 2:6 This article is available from: /1471-2148/2/6 © 2002 An et al; licensee BioMed Central Ltd. Verbatim copying and redistribution of this article are permitted in any medium for any purpose, provided this notice is preserved along with the articles original URL. Abstract Background: In fungi, aminoadipate reductase converts 2-aminoadipate to 2-aminoadipate 6- semialdehyde. However, other organisms have no homologue to the aminoadipate reductase gene and this pathway appears to be restricted to fungi. In this study, we designed degenerate primers for polymerase chain reaction (PCR) amplification of a large fragment of the aminoadipate reductase gene for divergent fungi. Results: Using these primers, we amplified DNA fragments from the archiascomycetous yeast Saitoella complicata and the black-ko