原创力文档an efficient method to successively introduce transgenes into a given genomic locus in the mouse一个有效的方法来给定基因组先后引入转基因在鼠标轨迹.pdfVIP
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an efficient method to successively introduce transgenes into a given genomic locus in the mouse一个有效的方法来给定基因组先后引入转基因在鼠标轨迹
BMC Developmental Biology (2001) 1:10 /1471-213X/1/10
BMC Developmental Biology (2001) 1:10
Methodology article
An efficient method to successively introduce transgenes into a
given genomic locus in the mouse
1 2 2 1
Roong Zhao , Scott A Fahs , Hartmut Weiler and Stephen A Duncan*
Address: 1Department of Cell Biology, Neurobiology, and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Rd, WI 53226, USA
and 2Blood Research Institute, The Blood Center of Southeastern Wisconsin, 8272 Watertown Plank Rd., Milwaukee, Wisconsin 53226
E-mail: Roong Zhao - rzhao@; Scott A Fahs - safahs@; Hartmut Weiler - hweiler@;
Stephen A Duncan* - duncans@
*Corresponding author
Published: 19 June 2001 Received: 3 May 2001
Accepted: 19 June 2001
BMC Developmental Biology 2001, 1:10
This article is available from: /1471-213X/1/10
(c) 2001 Zhao et al, licensee BioMed Central Ltd.
Abstract
Background: Expression of transgenes in mice requires transcriptional regulatory elements that
direct expression in a chosen cell type. Unfortunately, the availability of well-characterized
promoters that direct bona-fide expression of transgenes in transgenic mice is limited. Here we
described a method that allows highly efficient targeting of transgenes to a preselected locus in ES
cells.
Results: A pgk-LoxP-Neo cassette was introduced into a desired genomic locus by homologous
recombination in ES cells. The pgk promoter was then removed from the targeted ES cells by Cre
recombinase thereby restoring t
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