原创力文档an investigation of horizontal transfer of feed introduced dna to the aerobic microbiota of the gastrointestinal tract of rats调查水平饲料引入dna转移到有氧胃肠道微生物群的老鼠.pdfVIP
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an investigation of horizontal transfer of feed introduced dna to the aerobic microbiota of the gastrointestinal tract of rats调查水平饲料引入dna转移到有氧胃肠道微生物群的老鼠
Nordgård et al. BMC Research Notes 2012, 5:170
/1756-0500/5/170
RESEARCH ARTICLE Open Access
An investigation of horizontal transfer of feed
introduced DNA to the aerobic microbiota of the
gastrointestinal tract of rats
1,2 3 4,6 1,2 5 1,2*
Lise Nordgård , Lorenzo Brusetti , Noura Raddadi , Terje Traavik , Beate Averhoff and Kaare Magne Nielsen
Abstract
Background: Horizontal gene transfer through natural transformation of members of the microbiota of the lower
gastrointestinal tract (GIT) of mammals has not yet been described. Insufficient DNA sequence similarity for
homologous recombination to occur has been identified as the major barrier to interspecies transfer of
chromosomal DNA in bacteria. In this study we determined if regions of high DNA similarity between the
genomes of the indigenous bacteria in the GIT of rats and feed introduced DNA could lead to homologous
recombination and acquisition of antibiotic resistance genes.
Results: Plasmid DNA with two resistance genes (nptI and aadA) and regions of high DNA similarity to 16S rRNA
and 23S rRNA genes present in a broad range of bacterial species present in the GIT, were constructed and added
to standard rat feed. Six rats, with a normal microbiota, were fed DNA containing pellets daily over four days
before sampling of the microbiota from the different GI compartments (stomach, small intestine, cecum and
colon). In addition, two rats were included as negative controls. Antibiotic resistant colonies growing on selective
media were screened for recombination with feed introduced DNA by PCR targeting unique sites in the putatively
recombined regions. No transformants were identified among 441 tested isolates.
Conclusion
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