analysis of detergent-free lipid rafts isolated from cd4+ t cell line interaction with antigen presenting cells promotes coalescing of lipid rafts分析不用洗涤剂的脂质筏与cd4 + t细胞与抗原呈递细胞线互动促进脂筏的形成.pdfVIP

Kennedy et al. Cell Communication and Signaling 2011, 9:31 /content/9/1/31 RESEARCH Open Access Analysis of detergent-free lipid rafts isolated from CD4+ T cell line: interaction with antigen presenting cells promotes coalescing of lipid rafts Colleen Kennedy1,2, Matthew D Nelson1,3 and Anil K Bamezai1* Abstract Background: Lipid rafts present on the plasma membrane play an important role in spatiotemporal regulation of cell signaling. Physical and chemical characterization of lipid raft size and assessment of their composition before, and after cell stimulation will aid in developing a clear understanding of their regulatory role in cell signaling. We have used visual and biochemical methods and approaches for examining individual and lipid raft sub-populations isolated from a mouse CD4+ T cell line in the absence of detergents. Results: Detergent-free rafts were analyzed before and after their interaction with antigen presenting cells. We provide evidence that the average diameter of lipid rafts isolated from un-stimulated T cells, in the absence of detergents, is less than 100 nm. Lipid rafts on CD4+ T cell membranes coalesce to form larger structures, after interacting with antigen presenting cells even in the absence of a foreign antigen. Conclusions: Findings presented here indicate that lipid raft coalescence occurs during cellular interactions prior to sensing a foreign antigen. Keywords: raft coalescence, CD4+ T cells, antigen presenting cells, electron microscopy, raft-ELISA Background membrane may provide an additional level of spatial Signals emanating from the plasma membrane have spa- segregation [18-26]. Ligand and receptor induced mole- tial and temporal components [1-5]. Spatial distribution