analysis of mitotic phosphorylation of borealin有丝分裂borealin的磷酸化的分析.pdfVIP

BMC Cell Biology BioMed Central Research article Open Access Analysis of mitotic phosphorylation of Borealin Harpreet Kaur, Andrew C Stiff, Dipali A Date and William R Taylor* Address: Department of Biological Sciences, University of Toledo, 2801 W. Bancroft Street, MS 601, Toledo, OH 43606, USA Email: Harpreet Kaur - hkaur@; Andrew C Stiff - stiff1970@; Dipali A Date - dipali.date@; William R Taylor* - william .taylor3@ * Corresponding author Published: 22 January 2007 Received: 6 October 2006 Accepted: 22 January 2007 BMC Cell Biology 2007, 8:5 doi:10.1186/1471-2121-8-5 This article is available from: /1471-2121/8/5 © 2007 Kaur et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: The main role of the chromosomal passenger complex is to ensure that Aurora B kinase is properly localized and activated before and during mitosis. Borealin, a member of the chromosomal passenger complex, shows increased expression during G2/M phases and is involved in targeting the complex to the centromere and the spindle midzone, where it ensures proper chromosome segregation and cytokinesis. Borealin has a consensus CDK1 phosphorylation site, threonine 106 and can be phosphorylated by Aurora B Kinase at serine 165 in vitro. Result