原创力文档analysis of transient phosphorylation-dependent protein-protein interactions in living mammalian cells using split-tev瞬态分析phosphorylation-dependent蛋白质-蛋白质之间的关系在生活使用split-tev哺乳动物细胞.pdfVIP
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analysis of transient phosphorylation-dependent protein-protein interactions in living mammalian cells using split-tev瞬态分析phosphorylation-dependent蛋白质-蛋白质之间的关系在生活使用split-tev哺乳动物细胞
BMC Biotechnology BioMed Central
Methodology article Open Access
Analysis of transient phosphorylation-dependent protein-protein
interactions in living mammalian cells using split-TEV
1,2 1 1 1
Michael C Wehr , Lisa Reinecke , Anna Botvinnik and Moritz J Rossner*
Address: 1Research Group Gene Expression, Max-Planck-Institute of Experimental Medicine, 37075 Göttingen, Germany and 2Apoptosis and
Proliferation Control Laboratory, Cancer Research UK, London Research Institute (LRI), UK
Email: Michael C Wehr - Michael.Wehr@.uk; Lisa Reinecke - lreinecke@em.mpg.de; Anna Botvinnik - botvinnik@em.mpg.de;
Moritz J Rossner* - rossner@em.mpg.de
* Corresponding author
Published: 13 July 2008 Received: 24 May 2007
Accepted: 13 July 2008
BMC Biotechnology 2008, 8:55 doi:10.1186/1472-6750-8-55
This article is available from: /1472-6750/8/55
© 2008 Wehr et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Regulated protein-protein interactions (PPIs) are pivotal molecular switches that
are important for the regulation of signaling processes within eukaryotic cells. Cellular signaling is
altered in various disease conditions
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