原创力文档analyzing the gene expression profile of pediatric acute myeloid leukemia with real-time pcr arrays分析儿童急性髓系白血病的基因表达谱与实时pcr数组.pdfVIP
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Yan-Fang et al. Cancer Cell International 2012, 12:40
/content/12/1/40
PRIMARY RESEARCH Open Access
Analyzing the gene expression profile of pediatric
acute myeloid leukemia with real-time PCR arrays
1 1 1 1 1 1 1 1 1
Tao Yan-Fang , Wu Dong , Pang Li , Zhao Wen-Li , Lu Jun , Wang Na , Wang Jian , Feng Xing , Li Yan-Hong ,
Ni Jian2 and Pan Jian1,2*
Abstract
Background: The Real-time PCR Array System is the ideal tool for analyzing the expression of a focused panel of
genes. In this study, we will analyze the gene expression profile of pediatric acute myeloid leukemia with real-time
PCR arrays.
Methods: Real-time PCR array was designed and tested firstly. Then gene expression profile of 11 pediatric AML
and 10 normal controls was analyzed with real-time PCR arrays. We analyzed the expression data with MEV (Multi
Experiment View) cluster software. Datasets representing genes with altered expression profile derived from cluster
analyses were imported into the Ingenuity Pathway Analysis Tool.
Results: We designed and tested 88 real-time PCR primer pairs for a quantitative gene expression analysis of key
genes involved in pediatric AML. The gene expression profile of pediatric AML is significantly different from normal
control; there are 19 genes up-regulated and 25 genes down-regulated in pediatric AML. To investigate possible
biological interactions of differently regulated genes, datasets representing genes with altered expression profile
were imported into the Ingenuity Pathway Analysis Tool. The results revealed 12 significant networks. Of these
networks, Cellular Development, Cellular Growth and Proliferation, Tumor Morphology was the highest rated
network with 36 focus molecules and the s
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