antimicrobial activities of the methanol extract and compounds from the twigs of dorstenia mannii (moraceae)甲醇提取物的抗菌活动和化合物的树枝dorstenia mannii(桑科).pdfVIP

Mbaveng et al. BMC Complementary and Alternative Medicine 2012, 12:83 /1472-6882/12/83 RESEARCH ARTICLE Open Access Antimicrobial activities of the methanol extract and compounds from the twigs of Dorstenia mannii (Moraceae) 1,2* 1,5* 3 2 3,4 Armelle T Mbaveng , Victor Kuete , Bathelemy Ngameni , Veronique P Beng , Bonaventure T Ngadjui , Jacobus J Marion Meyer5 and Namrita Lall5 Abstract Background: Dorstenia mannii (Moraceae) is a medicinal herb used traditionally for the treatment of many diseases. In the present study, the methanol extract of D. mannii and nine of its isolated compounds, namely dorsmanin A (1), B (2), C (3), D (4), E (6), F (7), G (8) dorsmanin I (9) and 6,8-diprenyleriodictyol (5), were tested for their antimicrobial activities against yeast, Mycobacteria and Gram-negative bacteria. Methods: The microplate alamar blue assay (MABA) and the broth microdilution method were used to determine the minimal inhibitory concentration (MIC) and minimal microbicidal concentration (MMC) of the above extract and compounds on a panel of bacterial species. Results: The results of the MIC determinations demonstrated that the methanol extract as well as compounds 3 and 8 were able to prevent the growth of all the fourteen studied microorganisms within the concentration range of 4 to 1024 μg/ml. The lowest MIC value for the methanol extract (64 μg/ml) was obtained on Candida albicans. The lowest value for individual compounds (4 μg/ml) was recorded with compounds 3 on Pseudomonas aeruginosa PA01 and 7 on Eschericia coli ATCC strain. The MIC values recorded with compounds 3 on P. aeruginosa PA01, 6 on C. albicans, 7 on P. aeruginosa PA01 and K. pneumoniae ATCC s