high molecular mass proteomics analyses of left ventricle from rats subjected to differential swimming training高分子量的蛋白质组学分析从大鼠左心室进行微分游泳训练.pdfVIP

Rocha et al. BMC Physiology 2012, 12:11 /1472-6793/12/11 RESEARCH ARTICLE Open Access High molecular mass proteomics analyses of left ventricle from rats subjected to differential swimming training 1,5 1 1 2 1 Luiz A O Rocha , Bernardo A Petriz , David H Borges , Ricardo J Oliveira , Rosangela V de Andrade , Gilberto B Domont4, Rinaldo W Pereira1,2 and Octávio L Franco1,3,5* Abstract Background: Regular exercises are commonly described as an important factor in health improvement, being directly related to contractile force development in cardiac cells. In order to evaluate the links between swimming exercise intensity and cardiac adaptation by using high molecular mass proteomics, isogenic Wistar rats were divided into four groups: one control (CG) and three training groups (TG’s), with low, moderate and high intensity of exercises. In order to evaluate the links between swimming exercise intensity and cardiac adaptation by using high molecular mass proteomics, isogenic Wistar rats were divided into four groups: one control (CG) and three training groups (TG’s), with low, moderate and high intensity of exercises. Results: Findings here reported demonstrated clear morphologic alterations, significant cellular injury and increased energy supplies at high exercise intensities. α-MyHC, as well proteins associated with mitochondrial oxidative metabolism were shown to be improved. α-MyHC expression increase 1.2 fold in high intensity training group when compared with control group. α-MyHC was also evaluated by real-time PCR showing a clear expression correlation with protein synthesis data increase in 8.48 fold in high intensity training group. Other myofibrillar protein, troponin , appea